Abstract |
The discovery of accessible markers of lymphoma may facilitate the development of antibody-based therapeutic strategies. Here, we describe the results of a chemical proteomic study, based on the in vivo biotinylation of vascular proteins in lymphoma-bearing mice followed by mass spectrometric and bioinformatic analysis, to discover proteins expressed at the tissue-blood border of disseminated B-cell lymphoma. From a list of 58 proteins, which were more than 10-fold up-regulated in nodal and extranodal lymphoma lesions compared with their levels in the corresponding normal host organs, we validated BST-2 as a novel vascular marker of B-cell lymphoma, using immunochemical techniques and in vivo biodistribution studies. Furthermore, targeting BST-2 with 2 independent monoclonal antibodies delayed lymphoma growth in a syngeneic mouse model of the disease. The results of this study delineate a strategy for the treatment of systemic B-cell lymphoma in humans and suggest that anti-BST-2 antibodies may facilitate pharmacodelivery approaches that target the tumor-stroma interface.
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Authors | Christoph Schliemann, Christoph Roesli, Haruhiko Kamada, Beatrice Borgia, Tim Fugmann, Wolfram Klapper, Dario Neri |
Journal | Blood
(Blood)
Vol. 115
Issue 3
Pg. 736-44
(Jan 21 2010)
ISSN: 1528-0020 [Electronic] United States |
PMID | 19903902
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, CD
- Antigens, Neoplasm
- BST2 protein, mouse
- Membrane Glycoproteins
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Topics |
- Algorithms
- Animals
- Antibodies, Monoclonal
(therapeutic use)
- Antigens, CD
(immunology, metabolism, physiology)
- Antigens, Neoplasm
(immunology, metabolism)
- Biotinylation
(physiology)
- Blood Vessels
(immunology, metabolism)
- Cell Line, Tumor
- Female
- Immunization, Passive
(methods)
- Lymphoma, B-Cell
(metabolism, pathology, therapy)
- Membrane Glycoproteins
(antagonists & inhibitors, immunology, metabolism, physiology)
- Mice
- Mice, Inbred BALB C
- Models, Biological
- Neoplasm Transplantation
(immunology, pathology)
- Protein Array Analysis
- Transplantation, Isogeneic
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