Platelet-activating factor in porcine Pseudomonas acute lung injury.

Abstract

We investigated the role of platelet-activating factor (PAF) in acute septic lung injury by examining the effects of the selective PAF antagonist SRI 63-675 and by measuring PAF in lung tissue in the porcine model. Four groups of pigs (15-25 kg) were studied: saline control (C, n = 5); Pseudomonas (Ps, n = 9), given 5 x 10(8) CFU/ml at 0.3 ml/20 kg/min intravenously over 1 hr; SRI (n = 3), given SRI 63-675 in a 40 mg/kg bolus; and SRI + Ps (n = 5). Ps infusion produced a fulminant lung injury characterized by a threefold increase in pulmonary arterial pressure at 30 min and persistent pulmonary hypertension (P less than 0.05 vs C), a significant (P less than 0.05 vs C) decrease in arterial oxygen tension (PaO2) from 60 min, a significant (P less than 0.05 vs C) increase in extravascular lung water (EVLW) from 120 min, and a significant (P less than 0.05 vs C) increase in albumin flux determined scintigraphically as slope index at 150-180 min. Systemic arterial pressure and cardiac index (CI) decreased significantly (P less than 0.05) in the Ps group vs C at 60 and 180 min, respectively. Bolus injection of SRI 63-675 at the time of Ps infusion blocked the early pulmonary hypertension, attenuated the early and late fall in PaO2, ameliorated the increase in EVLW, and prevented the late (150-180 min) increase in albumin flux. SRI 63-675 had minimal effects on Ps-induced hypotension or alterations in CI.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: K Byrne, C N Sessler, P D Carey, T D Sielaff, A Vasquez, J L Tatum, J I Hirsch, H J Sugerman
• Journal: The Journal of surgical research (J Surg Res) Vol. 50 Issue 2 Pg. 111-8 (Feb 1991) ISSN: 0022-4804 [Print] United States
• PMID: 1990214 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Platelet Activating Factor
• Quinolines
• SRI 63-675

Topics

• Analysis of Variance
• Animals
• Blood Pressure (drug effects)
• Hemodynamics (drug effects)
• Lung Diseases (physiopathology)
• Platelet Activating Factor (analysis, antagonists & inhibitors, physiology)
• Pseudomonas Infections (physiopathology)
• Pulmonary Artery (drug effects, physiopathology)
• Quinolines (pharmacology)
• Swine