Abstract |
Blood-stage malaria parasites ablate memory B cells generated by vaccination in mice, resulting in diminishing natural boosting of vaccine-induced antibody responses to infection. Here we show the development of a new vaccine comprising a baculovirus-based Plasmodium yoelii 19-kDa carboxyl terminus of merozoite surface protein 1 (PyMSP1(19)) capable of circumventing the tactics of parasites in a murine model. The baculovirus-based vaccine displayed PyMSP1(19) on the surface of the virus envelope in its native three-dimensional structure. Needle-free intranasal immunization of mice with the baculovirus-based vaccine induced strong systemic humoral immune responses with high titers of PyMSP1(19)-specific antibodies. Most importantly, this vaccine conferred complete protection by natural boosting of vaccine-induced PyMSP1(19)-specific antibody responses shortly after challenge. The protective mechanism is a mixed Th1/Th2-type immunity, which is associated with the Toll-like receptor 9 (TLR9)-dependent pathway. The present study offers a novel strategy for the development of malaria blood-stage vaccines capable of naturally boosting vaccine-induced antibody responses to infection.
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Authors | Shigeto Yoshida, Hitomi Araki, Takashi Yokomine |
Journal | Infection and immunity
(Infect Immun)
Vol. 78
Issue 2
Pg. 595-602
(Feb 2010)
ISSN: 1098-5522 [Electronic] United States |
PMID | 19901059
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Protozoan
- Malaria Vaccines
- Merozoite Surface Protein 1
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Topics |
- Administration, Intranasal
- Animals
- Antibodies, Protozoan
(blood, immunology)
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- Female
- Fluorescent Antibody Technique
- Malaria
(prevention & control)
- Malaria Vaccines
(administration & dosage, immunology)
- Merozoite Surface Protein 1
(immunology)
- Mice
- Mice, Inbred BALB C
- Microscopy, Immunoelectron
- Plasmodium yoelii
(immunology)
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