Identification of proteins differentially expressed between capillary endothelial cells of hepatocellular carcinoma and normal liver in an orthotopic rat tumor model using 2-D DIGE.
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| Abstract |
Hepatocellular carcinoma (HCC) is one of the deadliest cancers with few treatment options. It is a hypervascular tumor in which angiogenesis plays a critical role in its progression. Tumor capillary endothelial cells (TECs) in HCC are known to originate from liver sinusoid endothelial cells, which then go through a capillarization process to become morphologically as well as functionally different TECs. In this work, we investigated proteins differentially expressed between freshly isolated TECs and sinusoid endothelial cells from well-formed rat HCC using 2-D DIGE coupled with MALDI-TOF/TOF MS. Thirty-eight unique proteins were identified to be differentially expressed more than twofold between the two endothelial cell types. Amongst the differentially expressed proteins, two novel endothelial markers, EH domain-containing protein 3 and galectin-3, were confirmed by Western blot and immunohistochemistry in both rat and human HCC samples. We showed that EH domain-containing protein 3 is significantly down-regulated in TECs, but galectin-3 is up-regulated. We propose possible roles of these two proteins in tumor vessel development in HCC.
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| Authors | Jinghui Jia, Jingyu Wang, Ming Teh, Wei Sun, Jianhua Zhang, Irene Kee, Pierce K-H Chow, Rosa Cynthia M-Y Liang, Maxey C M Chung, Ruowen Ge |
| Journal | Proteomics (Proteomics) Vol. 10 Issue 2 Pg. 224-34 (Jan 2010) ISSN: 1615-9861 [Electronic] Germany |
| PMID | 19899081 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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