Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells.

Abstract

Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC(50) value of cimiside E on gastric cancer cells for 24 h was 14.58 microM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 microM) and G2/M phase at higher concentrations (60 and 90 microM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.

• Authors: Lian Yu Guo, Eun Ji Joo, Kun Ho Son, Su Jin Jeon, Sehyun Jang, Eun Myoung Shin, Hong Yu Zhou, Yeong Shik Kim
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 32 Issue 10 Pg. 1385-92 (Oct 2009) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 19898801 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Annexin A5
• Antineoplastic Agents, Phytogenic
• FAS protein, human
• FASLG protein, human
• Fas Ligand Protein
• Saponins
• Triterpenes
• cimiside E
• fas Receptor

Topics

• Annexin A5 (metabolism)
• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cimicifuga (chemistry)
• DNA Fragmentation (drug effects)
• Fas Ligand Protein (metabolism)
• Humans
• Reverse Transcriptase Polymerase Chain Reaction
• Saponins (isolation & purification, pharmacology)
• Stomach Neoplasms (pathology)
• Triterpenes (isolation & purification, pharmacology)
• fas Receptor (metabolism)