Prognostic value of biochemical markers of brain damage and oxidative stress in post-surgical aneurysmal subarachnoid hemorrhage patients.

Abstract

The aim of this study is to determine effective biochemical markers and optimal sampling timing for prediction of neurological prognosis in post-surgical aneurysmal subarachnoid hemorrhage (SAH) patients. Subjects were a sequential group of SAH patients admitted to our centre who underwent aneurysm clipping before Day 3 and who received a cerebrospinal fluid (CSF) drain. CSF samples from 32 patients were collected on Days 3, 7, and 14. Neurological outcome was assessed by neurosurgeons using the Glasgow outcome scale (GOS) at 6 months after onset. CSF levels of neuron-specific enolase (NSE), S100B, and glial fibrillary acidic protein (GFAP) were determined using enzyme-linked immunosorbent assay, and the CSF concentrations of malondialdehyde (MDA) were determined using spectrophotometric assay. In univariate analysis, S100B on Days 3 and 14, GFAP on Days 3 and 7, and MDA on Day 14 were significantly higher in the poor outcome group (GOS 1-4) than in the good outcome group (GOS 5). In multivariate analysis, only MDA on Day 14 was identified as a significant predictor of poor neurological outcome at 6 months after onset. The area under the receiver-operating characteristic (ROC) curve for MDA on Day 14 was 0.841. For a threshold of 0.3 microM, sensitivity and specificity were 0.875 and 0.750, respectively. Our findings suggest that these biochemical markers, especially MDA, show significant promise as predictors of neurological outcome in clinical practice.

• Authors: Kotaro Kaneda, Motoki Fujita, Susumu Yamashita, Tadashi Kaneko, Yoshikatsu Kawamura, Tomonori Izumi, Ryosuke Tsuruta, Shunji Kasaoka, Tsuyoshi Maekawa
• Journal: Brain research bulletin (Brain Res Bull) Vol. 81 Issue 1 Pg. 173-7 (Jan 15 2010) ISSN: 1873-2747 [Electronic] United States
• PMID: 19887101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glial Fibrillary Acidic Protein
• Nerve Growth Factors
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins
• S100B protein, human
• Malondialdehyde
• Phosphopyruvate Hydratase

Topics

• Analysis of Variance
• Area Under Curve
• Brain (pathology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Glial Fibrillary Acidic Protein (cerebrospinal fluid)
• Humans
• Intracranial Aneurysm (cerebrospinal fluid, diagnosis, etiology)
• Male
• Malondialdehyde (cerebrospinal fluid)
• Middle Aged
• Nerve Growth Factors (cerebrospinal fluid)
• Oxidative Stress
• Phosphopyruvate Hydratase (cerebrospinal fluid)
• Prognosis
• ROC Curve
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins (cerebrospinal fluid)
• Sensitivity and Specificity
• Severity of Illness Index
• Spectrophotometry
• Subarachnoid Hemorrhage (cerebrospinal fluid, diagnosis, etiology)
• Time Factors