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Induction of matrix metalloproteinase-9 by galectin-7 through p38 MAPK signaling in HeLa human cervical epithelial adenocarcinoma cells.

Abstract
The expression of matrix metalloproteinase-9 (MMP-9) has been reported in various cancers. Its expression is associated with tumorigenesis and tumor metastasis. Studies have shown that galectin-7, a beta-galactoside-binding animal lectin, is involved in various processes including the suppression of tumor growth. However, a recent study reported that the development of thymic lymphoma is accelerated by galectin-7 expression. In this report, we demonstrate that the expression of MMP-9 was increased by galectin-7 in human cervix epithelial cells (HeLa). When the galectin-7 gene was transfected to HeLa cells with the ECFP vector, the expression of MMP-9 mRNA increased, as compared to non-transfected cells. As a result, MMP-9 protein levels also increased, as indicated by Western blot and gelatin zymography. In addition, galectin-7-transfected cells exhibited increased invasion in the matrigel invasion system. Expression of MMP-9 is involved in several signaling pathways by various stimulation factors. Therefore, we investigated how the signaling pathway in galectin-7-transfected cells differs from that of non-transfected cells. Upon transfection of galectin-7, p38 MAPK was activated and SB203580, a chemical inhibitor of p38 MAPK, reversed the effects of galectin-7. These results indicate that galectin-7 increases the expression of MMP-9 through the p38 MAPK signaling pathway.
AuthorsJi E Park, Weon Y Chang, Moonjae Cho
JournalOncology reports (Oncol Rep) Vol. 22 Issue 6 Pg. 1373-9 (Dec 2009) ISSN: 1791-2431 [Electronic] Greece
PMID19885589 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Carbohydrates
  • Enzyme Inhibitors
  • Galectins
  • LGALS7 protein, human
  • p38 Mitogen-Activated Protein Kinases
  • Matrix Metalloproteinase 9
Topics
  • Carbohydrates (chemistry)
  • Cell Line, Tumor
  • Enzyme Inhibitors (pharmacology)
  • Female
  • Galectins (metabolism)
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Neoplastic
  • HeLa Cells
  • Humans
  • Matrix Metalloproteinase 9 (metabolism)
  • Microscopy, Fluorescence (methods)
  • Neoplasm Invasiveness
  • Protein Binding
  • Uterine Cervical Neoplasms (metabolism)
  • p38 Mitogen-Activated Protein Kinases (metabolism)

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