Bisabolangelone isolated from Ostericum koreanum inhibits the production of inflammatory mediators by down-regulation of NF-kappaB and ERK MAP kinase activity in LPS-stimulated RAW264.7 cells.

Abstract

Bisabolangelone, a sesquiterpene derivative, was isolated from the roots of Osterici Radix (Ostericum koreanum Maximowicz). In this study, the anti-inflammatory effect of bisabolangelone was investigated to address potential therapeutic effects in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells. Bisabolangelone significantly inhibited NO, PGE(2), and pro-inflammatory cytokines by suppressing the mRNA and protein expressions of iNOS and COX-2. Bisabolangelone also inhibited the productions of pro-inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) by suppressing the cytokine mRNA and protein expressions. The molecular mechanism of bisabolangelone-mediated attenuation in RAW 264.7 cells has a close relationship to suppressing the translocation of nuclear factor-kappaB (NF-kappaB) p65 subunit into the nucleus and the phosphorylation of mitogen-activated protein kinases (MAPKs). These results indicate that bisabolangelone inhibits LPS-stimulated inflammation through the blocking of NF-kappaB and MAPK pathways in macrophages, and demonstrated that bisabolangelone possesses anti-inflammatory properties.

• Authors: Hyo Won Jung, Ramalingam Mahesh, Jun Hong Park, Yong Chool Boo, Kwon Moo Park, Yong-Ki Park
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 10 Issue 2 Pg. 155-62 (Feb 2010) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 19879381 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Cyclooxygenase 2 Inhibitors
• Cytokines
• Inflammation Mediators
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• Sesquiterpenes
• bisabolangelone
• Nitric Oxide
• Extracellular Signal-Regulated MAP Kinases
• Dinoprostone

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (isolation & purification, pharmacology)
• Apiaceae (chemistry)
• Cell Line
• Cyclooxygenase 2 Inhibitors (therapeutic use)
• Cytokines (antagonists & inhibitors)
• Dinoprostone (antagonists & inhibitors)
• Down-Regulation (drug effects)
• Extracellular Signal-Regulated MAP Kinases (antagonists & inhibitors)
• Inflammation (drug therapy)
• Inflammation Mediators (antagonists & inhibitors)
• Lipopolysaccharides (antagonists & inhibitors)
• Macrophages (drug effects)
• Mice
• NF-kappa B (antagonists & inhibitors)
• Nitric Oxide (antagonists & inhibitors)
• Plant Extracts (isolation & purification)
• Plant Roots (chemistry)
• Sesquiterpenes (isolation & purification, pharmacology)