[Effects of PCB153 on oxidative stress and 8-OHdG content induced by PBDE-47 in human neuroblastoma cells in vitro].

Abstract

OBJECTIVE:

To explore the effects of PCB153 (2,2,4,4,5,5-hexachlorobiphenyl) on oxidative stress and 8-OHdG (8-hydroxy-2'-deoxyguanosine) content induced by PBDE-47 (2,2,4,4-tetrabromodiphenyl ether) in SH-SY5Y cells.

METHODS:

SH-SY5Y cells were incubated with different concentrations of 1, 5, 10 micromol/L PBDE-47 or/and 5 micromol/L PCB153 and antioxidant N-acetyl cysteine (NAC 100 micromol/L) for 24h in vitro, ROS (reactive oxygen species) level and 8-OHdG were measured by using DCFH-DA fluorescent marking method and the skill of high performance liquid chromatogram-electrochemistry (HPLC-EC), respectively.

RESULTS:

As for ROS formation, there was no significant difference in PBDE-47 groups alone compared to the control group and their corresponding combined groups, respectively (P > 0.05). ROS formation were significantly increased in 5 and 10 micromol/L combined groups compared to the control group and their corresponding concentrations of PBDE-47 groups or PCB153 group, respectively (P < 0.05). 8-OHdG levels were significantly increased in 10 micromol/L PBDE-47, 5 micromol/L PBDE-47 + 5 micromol/L PCB153, and 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups compared with the control (P < 0.05). 8-OHdG level was dramatically increased in 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups compared to their corresponding doses of PBDE-47 groups or PCB153 group (P < 0.05). The groups coincubation with N-acetylcysteine caused a decrease in cellular ROS level and ameliorated PBDE-47-mediated oxidative DNA damage effects. Positive relationship was observed between ROS level and 8-OHdG contents (r = 0.895, P < 0.01).

CONCLUSION:

These results provide evidence of a direct or indirect relationship between PBDE-47 and oxidative DNA damage. Furthermore, PBDE-47 combined with PCB153 may increase the effects on oxidative DNA damage in SH-SY5Y cells in vitro, oxidative stress may responsible for DNA damage induced by PBDE-47.

• Authors: Ping Gao, Ping He, Aiguo Wang, Tao Xia, Zhixia Xu, Qiang Niu, Lijuan Guo, Xuemin Chen
• Journal: Wei sheng yan jiu = Journal of hygiene research (Wei Sheng Yan Jiu) Vol. 38 Issue 5 Pg. 513-5 (Sep 2009) ISSN: 1000-8020 [Print] China
• PMID: 19877502 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Halogenated Diphenyl Ethers
• Reactive Oxygen Species
• 2,2',4,4'-tetrabromodiphenyl ether
• 8-Hydroxy-2'-Deoxyguanosine
• Polychlorinated Biphenyls
• Deoxyguanosine
• 2,4,5,2',4',5'-hexachlorobiphenyl

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Cell Line, Tumor
• DNA Damage (drug effects)
• Deoxyguanosine (analogs & derivatives, metabolism)
• Halogenated Diphenyl Ethers (toxicity)
• Humans
• Neuroblastoma (metabolism, pathology)
• Oxidative Stress (drug effects)
• Polychlorinated Biphenyls (toxicity)
• Reactive Oxygen Species (metabolism)