Abstract | OBJECTIVES: The aim of this study was to investigate the effect of levosimendan on apoptosis and infarct size when administered before ischemia in an isolated rat heart model. DESIGN: An in vitro experimental study. SETTING: Animal laboratory. PARTICIPANTS: Isolated perfused rat heart preparation (n = 22). INTERVENTIONS: Perfusion with Krebs-Henseleit solution was performed for 30 minutes and then 0.1 micromol/L of levosimendan was added to the perfusion fluid for 10 minutes before global ischemia; the control hearts received no levosimendan. Hearts underwent global ischemia for 30 minutes and then were reperfused for 30 minutes before specimens were obtained for testing. MEASUREMENTS AND MAIN RESULTS:
Infarct sizes were measured at the end of the reperfusion period and expressed as a percentage of the area at risk. Myocardial apoptosis was detected by using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) method. Bcl-2 expression was determined to detect antiapoptotic activity. Infarct size was significantly less in the levosimendan group (26% +/- 3% v 40% +/- 4%, respectively; p = 0.009). Levosimendan significantly reduced the proportion of TUNEL-positive cardiomyocytes (3 +/- 1 v 20 +/- 4, respectively; p < 0.001) and increased Bcl-2 expression compared with control hearts (44% +/- 3% v 31% +/- 3%, respectively; p = 0.01). Recovery of left ventricular-developed pressure 30 minutes after reperfusion in ischemic hearts pretreated with levosimendan was significantly better than that of placebo-treated hearts (53% +/- 3% v 38% +/- 3% of baseline, respectively; p = 0.004). CONCLUSIONS:
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Authors | Tulun Ozturk, Sule Gok, Nalan Nese |
Journal | Journal of cardiothoracic and vascular anesthesia
(J Cardiothorac Vasc Anesth)
Vol. 24
Issue 4
Pg. 624-8
(Aug 2010)
ISSN: 1532-8422 [Electronic] United States |
PMID | 19875306
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Hydrazones
- Pyridazines
- Simendan
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Topics |
- Animals
- Apoptosis
(drug effects, physiology)
- Disease Models, Animal
- Hydrazones
(therapeutic use)
- Male
- Myocardial Reperfusion Injury
(pathology, prevention & control)
- Organ Culture Techniques
- Pyridazines
(therapeutic use)
- Rats
- Rats, Wistar
- Simendan
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