Abstract | PURPOSE: To compare fibulin-3 (FIB-3) and fibulin-5 (FIB-5) expressions in uterosacral ligaments (USL) of women with and without uterine prolapse. STUDY DESIGN: USL were sampled in a standardized fashion from women with (n = 8) or without (n = 8) uterine prolapse. Quantitative Real-Time PCR was performed to measure mRNA and immunohistochemistry to assess protein expression. RESULTS: FIB-3 mRNA expression and FIB-3 staining intensity was similar in the USL of women with and without uterine prolapse {[(FIB-3 mean +/- SD mRNA relative units) 0.45 +/- 0.41 vs. 0.46 +/- 0.82, NS] and [Intensity score, median (range), 1(0-1) vs. 1(0-1), NS]}. Both FIB-5 mRNA expression and FIB-5 staining intensity was significantly decreased in USL from women with uterine prolapse compared to women without prolapse {[(FIB-5 mean +/- SD mRNA relative units) 0.07 +/- 0.02 vs. 0.26 +/- 0.20, P = 0.02] and [Intensity score, median (range), 0(0-2) vs. 3(2-3), P = 0.002]}. CONCLUSION:
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Authors | Peter Takacs, Mehdi Nassiri, Keith Candiotti, Jinfeng Yang, Sujata Yavagal, Carlos A Medina |
Journal | Archives of gynecology and obstetrics
(Arch Gynecol Obstet)
Vol. 282
Issue 4
Pg. 389-94
(Oct 2010)
ISSN: 1432-0711 [Electronic] Germany |
PMID | 19862539
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- EFEMP1 protein, human
- Extracellular Matrix Proteins
- FBLN5 protein, human
- RNA, Messenger
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Topics |
- Adult
- Case-Control Studies
- Extracellular Matrix Proteins
(metabolism)
- Female
- Florida
- Humans
- Immunohistochemistry
- Ligaments
(metabolism)
- Middle Aged
- Polymerase Chain Reaction
- RNA, Messenger
(genetics, metabolism)
- Sacrum
(metabolism)
- Uterine Prolapse
(genetics, metabolism)
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