Abstract |
In spite of the development of new anticancer drugs by the pharmaceutical industry, melanoma and T lymphomas are diseases for which medical advances remain limited. Thus, there was an urgent need of new therapeutics with an original mechanism of action. Since several years, our group develops quinoxalinic compounds. In this paper, the first preclinical results concerning one lead compound, EAPB0203, are presented. This compound exhibits in vitro cytotoxic activity on A375 and M4Be human melanoma cell lines superior to that of imiquimod and fotemustine. A liquid chromatography-mass spectrometry method was first validated to simultaneously quantify EAPB0203 and its metabolite, EAPB0202, in rat plasma. Thereafter, the pharmacokinetic profiles of EAPB0203 were studied in rat after intravenous and intraperitoneal administrations. After intraperitoneal administration the absolute bioavailability remains limited (22.7%). In xenografted mouse, after intraperitoneal administration of 5 and 20mg/kg, EAPB0203 is more potent than fotemustine. The survival time was increased up to 4 and 2 weeks compared to control mice and mice treated by fotemustine, respectively. The results of this study demonstrate the relationship between the dose of EAPB0203 and its effects on tumor growth. Thus, promising efficacy, tolerance and pharmacokinetic data of EAPB0203 encourage the development towards patient benefit.
|
Authors | Sonia Khier, Carine Deleuze-Masquéfa, Georges Moarbess, Florence Gattacceca, Delphine Margout, Isabelle Solassol, Jean-François Cooper, Frédéric Pinguet, Pierre-Antoine Bonnet, Françoise M M Bressolle |
Journal | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
(Eur J Pharm Sci)
Vol. 39
Issue 1-3
Pg. 23-9
(Jan 31 2010)
ISSN: 1879-0720 [Electronic] Netherlands |
PMID | 19854270
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright 2009 Elsevier B.V. All rights reserved. |
Chemical References |
- 1-(2-phenethyl)imidazo(1,2-a)quinoxalin-4-amine
- Aminoquinolines
- Antineoplastic Agents
- EAPB0203
- Imidazoles
- Nitrosourea Compounds
- Organophosphorus Compounds
- Quinoxalines
- fotemustine
- Imiquimod
|
Topics |
- Aminoquinolines
(pharmacology)
- Animals
- Antineoplastic Agents
(pharmacokinetics, pharmacology)
- Cell Line, Tumor
- Dose-Response Relationship, Drug
- Humans
- Imidazoles
(pharmacokinetics)
- Imiquimod
- Injections, Intraperitoneal
- Injections, Intravenous
- Male
- Melanoma
(drug therapy)
- Mice
- Mice, Nude
- Nitrosourea Compounds
(pharmacology)
- Organophosphorus Compounds
(pharmacology)
- Quinoxalines
(administration & dosage, pharmacokinetics, pharmacology)
- Rats
- Skin Neoplasms
(drug therapy)
- Xenograft Model Antitumor Assays
|