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Effect of 5-S-GAD on UV-B-induced cataracts in rats.

AbstractPURPOSE:
5-S-Glutathionyl-N-beta-alanyl-3,4-dihydroxyphenylalanine (5-S-GAD) is a novel antibacterial substance purified from Sarcophaga peregrina (flesh fly) that has both a radical scavenging activity and antioxidative activity. This is a report of an investigation of the effect of 5-S-GAD (eyedrops) on UVB-induced cataracts in rats.
METHODS:
Brown Norway male rats (n = 32; 7 weeks old) were treated with either 5-S-GAD 0.1%, 5-SGAD 1%, astaxanthin (AST) 0.1% suspension eyedrops or the vehicle alone (the solution without 5-S-GAD) three times a day (three doses at 5-min intervals each time). The treatment was scheduled 2 days before UV-B exposure and 2 days after UV-B exposure. Exposure to 100-200 mJ/cm(2) UV-B was performed once a week between drug treatments for 9 consecutive weeks, with a total dose of 1200 mJ/cm(2) UV-B. Ocular penetration of 5-S-GAD was analyzed using high-pressure liquid chromatography (HPLC). Cataract formation was documented by an anterior eye segment analysis system once a week under mydriasis. The light-scattering intensity (LSI) of the anterior superficial cortex region was measured.
RESULTS:
In the eighth to ninth week after the start of UV-B exposure, the LSI of anterior subcapsular lenses of 5-S-GAD-treated groups, as detected by HPLC, was significantly lower (P < 0.05) than that of the control, whereas no such difference was found in the AST-treated group.
CONCLUSION:
5-S-GAD eyedrop application may delay the progression of UV-B-induced cataract in rats.
AuthorsHiroyoshi Kawada, Masami Kojima, Takahito Kimura, Shunji Natori, Kazuyuki Sasaki, Hiroshi Sasaki
JournalJapanese journal of ophthalmology (Jpn J Ophthalmol) Vol. 53 Issue 5 Pg. 531-5 (Sep 2009) ISSN: 1613-2246 [Electronic] Japan
PMID19847611 (Publication Type: Journal Article)
Chemical References
  • Antioxidants
  • Free Radical Scavengers
  • N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine
  • Ophthalmic Solutions
  • Dihydroxyphenylalanine
  • Protein-Tyrosine Kinases
  • Glutathione
Topics
  • Administration, Topical
  • Animals
  • Antioxidants (administration & dosage, pharmacokinetics)
  • Cataract (drug therapy, etiology, physiopathology)
  • Chromatography, High Pressure Liquid
  • Dihydroxyphenylalanine (administration & dosage, analogs & derivatives, pharmacokinetics)
  • Disease Models, Animal
  • Free Radical Scavengers (administration & dosage, pharmacokinetics)
  • Glutathione (administration & dosage, analogs & derivatives, pharmacokinetics)
  • Lens, Crystalline (physiopathology, radiation effects)
  • Light
  • Male
  • Ophthalmic Solutions
  • Protein-Tyrosine Kinases (antagonists & inhibitors)
  • Radiation Injuries, Experimental (drug therapy, etiology)
  • Rats
  • Rats, Inbred BN
  • Scattering, Radiation
  • Ultraviolet Rays (adverse effects)

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