Abstract | PURPOSE: 5-S-Glutathionyl-N-beta-alanyl-3,4-dihydroxyphenylalanine (5-S-GAD) is a novel antibacterial substance purified from Sarcophaga peregrina (flesh fly) that has both a radical scavenging activity and antioxidative activity. This is a report of an investigation of the effect of 5-S-GAD ( eyedrops) on UVB-induced cataracts in rats. METHODS: Brown Norway male rats (n = 32; 7 weeks old) were treated with either 5-S-GAD 0.1%, 5-SGAD 1%, astaxanthin (AST) 0.1% suspension eyedrops or the vehicle alone (the solution without 5-S-GAD) three times a day (three doses at 5-min intervals each time). The treatment was scheduled 2 days before UV-B exposure and 2 days after UV-B exposure. Exposure to 100-200 mJ/cm(2) UV-B was performed once a week between drug treatments for 9 consecutive weeks, with a total dose of 1200 mJ/cm(2) UV-B. Ocular penetration of 5-S-GAD was analyzed using high-pressure liquid chromatography (HPLC). Cataract formation was documented by an anterior eye segment analysis system once a week under mydriasis. The light-scattering intensity (LSI) of the anterior superficial cortex region was measured. RESULTS: In the eighth to ninth week after the start of UV-B exposure, the LSI of anterior subcapsular lenses of 5-S-GAD-treated groups, as detected by HPLC, was significantly lower (P < 0.05) than that of the control, whereas no such difference was found in the AST-treated group. CONCLUSION:
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Authors | Hiroyoshi Kawada, Masami Kojima, Takahito Kimura, Shunji Natori, Kazuyuki Sasaki, Hiroshi Sasaki |
Journal | Japanese journal of ophthalmology
(Jpn J Ophthalmol)
Vol. 53
Issue 5
Pg. 531-5
(Sep 2009)
ISSN: 1613-2246 [Electronic] Japan |
PMID | 19847611
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Free Radical Scavengers
- N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine
- Ophthalmic Solutions
- Dihydroxyphenylalanine
- Protein-Tyrosine Kinases
- Glutathione
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Topics |
- Administration, Topical
- Animals
- Antioxidants
(administration & dosage, pharmacokinetics)
- Cataract
(drug therapy, etiology, physiopathology)
- Chromatography, High Pressure Liquid
- Dihydroxyphenylalanine
(administration & dosage, analogs & derivatives, pharmacokinetics)
- Disease Models, Animal
- Free Radical Scavengers
(administration & dosage, pharmacokinetics)
- Glutathione
(administration & dosage, analogs & derivatives, pharmacokinetics)
- Lens, Crystalline
(physiopathology, radiation effects)
- Light
- Male
- Ophthalmic Solutions
- Protein-Tyrosine Kinases
(antagonists & inhibitors)
- Radiation Injuries, Experimental
(drug therapy, etiology)
- Rats
- Rats, Inbred BN
- Scattering, Radiation
- Ultraviolet Rays
(adverse effects)
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