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Exogenous expression of caspase-14 induces tumor suppression in human salivary cancer cells by inhibiting tumor vascularization.

AbstractBACKGROUND:
Current therapeutic approaches to salivary gland cancer are often associated with severe disfigurement and loss of glandular function, which are traumatic to the patients. Exploration of novel treatment approaches, such as gene therapy, is needed.
MATERIALS AND METHODS:
The human salivary gland cancer cell line HSG was transiently transfected with full length human caspase-14 cDNA. Photomicroscopy, BrdU assay, cell counting, MTT assay, and TUNEL assay were applied. To determine the tumorigenicity, tumor volume, tumor pathology and vascularization were analyzed in vivo.
RESULTS:
Cell growth and viability were inhibited significantly by transient caspase-14 expression. Caspase-14 expression resulted in a significant reduction of tumorigenicity. Importantly, a significant decrease in tumor blood vessel formation was observed.
CONCLUSION:
Salivary gland cancer cells underwent growth inhibition, cell death, and reduced tumorigenicity in vivo when exogenous caspase-14 was expressed, which could be due, in part, to an inhibitory effect of caspase-14 on tumor vascularization.
AuthorsMengjie Wu, Isamu Kodani, Douglas Dickinson, Frank Huff, Kalu U E Ogbureke, Haiyan Qin, Senthil Arun, Rachel Dulebohn, Mohamed Al-Shabrawey, Amany Tawfik, Susan Prater, Jill Lewis, John Wataha, Regina Messer, Stephen Hsu
JournalAnticancer research (Anticancer Res) Vol. 29 Issue 10 Pg. 3811-8 (Oct 2009) ISSN: 1791-7530 [Electronic] Greece
PMID19846913 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Neoplasm
  • Caspase 14
Topics
  • Adenocarcinoma (blood supply, enzymology, genetics, therapy)
  • Animals
  • Caspase 14 (biosynthesis, genetics, metabolism)
  • Cell Growth Processes (genetics)
  • Cell Line, Tumor
  • DNA, Neoplasm (biosynthesis, genetics)
  • Female
  • Genetic Therapy (methods)
  • Genetic Vectors (genetics)
  • Humans
  • Mice
  • Mice, Nude
  • Neovascularization, Pathologic (enzymology, genetics, pathology)
  • Plasmids (genetics)
  • Salivary Gland Neoplasms (blood supply, enzymology, genetics, therapy)
  • Transfection
  • Xenograft Model Antitumor Assays

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