3-aminobenzamide, a poly ADP ribose polymerase inhibitor, attenuates renal ischemia/reperfusion injury.

Abstract

INTRODUCTION:

This study was designed to investigate whether 3-amino benzamide (3-AB), a poly (ADP-ribose) polymerase (PARP) inhibitor, has a protective effect on kidney injury induced by renal ischemia/reperfusion (I/R) by decreasing oxidative and nitrosative stress on renal dysfunction and injury.

MATERIALS AND METHODS:

Thirty-two male Sprague-Dawley rats were divided into four groups: sham-operated, sham-operated + 3-AB, I/R, I/R + 3-AB. Rats were given 3-AB (100 mg/kg/day ip) 14 days prior to I/R. I/R and I/R + 3-AB groups underwent 60 min of bilateral renal ischemia followed by 6 h of reperfusion. After reperfusion, kidneys and blood were obtained for evaluation. Superoxide dismutase, glutathione peroxidase, malondialdehide, protein carbonyl content, and nitrite/nitrate level (NO(x)) were determined in the renal tissue. Serum creatinine (S(Cr)), blood urea nitrogen (BUN), and aspartate aminotransferase (AST) were determined in the blood. Additionally, renal sections were used for histological grade of renal injury.

RESULTS:

3-AB significantly reduced the I/R-induced increases in S(Cr), BUN, and AST. In addition, 3-AB markedly reduced elevated oxidative stress product, restored decreased antioxidant enzymes, and attenuated histological alterations. Moreover, 3-AB attenuated the tissue NO(x) levels, indicating reduced NO production.

CONCLUSIONS:

3-AB has beneficial effect on renal glomerular and tubular dysfunction in rats' kidneys subjected to I/R injury. Moreover, 3-AB has ameliorating effect on both oxidative stress and nitrosative stress of the kidneys, which correlated with histopathological evaluation.

• Authors: Emin Oztas, Ahmet Guven, Erdal Turk, Bulent Uysal, Emin Ozgur Akgul, Tuncer Cayci, Nail Ersoz, Ahmet Korkmaz
• Journal: Renal failure (Ren Fail) Vol. 31 Issue 5 Pg. 393-9 ( 2009) ISSN: 1525-6049 [Electronic] England
• PMID: 19839840 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Benzamides
• Enzyme Inhibitors
• 3-aminobenzamide
• Creatinine

Topics

• Animals
• Benzamides (pharmacology)
• Blood Chemical Analysis
• Blood Urea Nitrogen
• Creatinine (blood)
• Disease Models, Animal
• Enzyme Inhibitors (pharmacology)
• Immunohistochemistry
• Infusions, Parenteral
• Kidney Function Tests
• Male
• Oxidative Stress (drug effects)
• Probability
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (pathology, prevention & control)
• Statistics, Nonparametric