The zymographic evaluation of gelatinase (MMP-2 and -9) levels in acute and chronic periapical abscesses.

Abstract

OBJECTIVE:

This study investigated the presence and levels of matrix metalloproteinases (MMP)-2 and -9 in periapical abscesses.

STUDY DESIGN:

Eighteen samples of intracanal exudates containing pus were collected from teeth with clinically and radiographically verified primary or secondary acute and chronic apical abscesses. Pro- and active forms of MMP-2 and MMP-9 levels were analyzed by using substrate gel zymography followed by an image analysis system. Statistical analysis was performed using the Kruskall-Wallis and Mann-Whitney U tests with Bonferroni adjustment.

RESULTS:

Both forms of MMP-9 were detected in all pus samples and demonstrated marked differences among the experimental groups (P < .05). Primary or secondary acute apical abscess samples demonstrated significantly higher MMP-9 levels compared with MMP-2 levels (P < .01). However, MMP-2 could not be detected in chronic apical abscesses.

CONCLUSION:

According to the results of this study, gelatinases might affect the pathogenesis of acute and chronic periapical abscesses.

• Authors: Hatice Dogan Buzoglu, Hasan Unal, Celal Ulger, Safak Mert, Sibel Kücükyildirim, Nuray Er
• Journal: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (Oral Surg Oral Med Oral Pathol Oral Radiol Endod) Vol. 108 Issue 5 Pg. e121-6 (Nov 2009) ISSN: 1528-395X [Electronic] United States
• PMID: 19836706 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Acute Disease
• Adolescent
• Adult
• Chronic Disease
• Electrophoresis, Polyacrylamide Gel
• Female
• Humans
• Male
• Matrix Metalloproteinase 2 (analysis)
• Matrix Metalloproteinase 9 (analysis)
• Middle Aged
• Periapical Abscess (enzymology)
• Suppuration
• Young Adult