Sodium tungstate attenuate oxidative stress in brain tissue of streptozotocin-induced diabetic rats.

Abstract

High blood glucose concentration in diabetes induces free radical production and, thus, causes oxidative stress. Damage of cellular structures by free radicals play an important role in development of diabetic complications. In this study, we evaluated effects of sodium tungstate on enzymatic and nonenzymatic markers of oxidative stress in brain of streptozotocin (STZ)-induced diabetic rats. Rats were divided into four groups (ten rats in each group): untreated control, sodium tungstate-treated control, untreated diabetic, and sodium tungstate-treated diabetic. Diabetes was induced with an intraperitoneal STZ injection (65 mg/kg body weight), and sodium tungstate with concentration of 2 g/L was added to drinking water of treated animals for 4 weeks. Diabetes caused a significant increase in the brain thiobarbituric acid reactive substances (P < 0.01) and protein carbonyl levels (P < 0.01) and a decrease in ferric reducing antioxidant power (P < 0.01). Moreover, diabetic rats presented a reduction in brain glucose-6-phosphate dehydrogenase (21%), superoxide dismutase (41%), glutathione peroxidase (19%), and glutathione reductase (36%) activities. Sodium tungstate reduced the hyperglycemia and restored the diabetes-induced changes in all mentioned markers of oxidative stress. However, catalase activity was not significantly affected by diabetes (P = 0.4), while sodium tungstate caused a significant increase in enzyme activity of treated animals (P < 0.05). Data of present study indicated that sodium tungstate can ameliorate brain oxidative stress in STZ-induced diabetic rats, probably by reducing of the high glucose-induced oxidative stress and/or increasing of the antioxidant defense mechanisms.

• Authors: Alireza Nakhaee, Mohammad Bokaeian, Azim Akbarzadeh, Mohammad Hashemi
• Journal: Biological trace element research (Biol Trace Elem Res) Vol. 136 Issue 2 Pg. 221-31 (Aug 2010) ISSN: 1559-0720 [Electronic] United States
• PMID: 19834650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Hypoglycemic Agents
• Tungsten Compounds
• sodium tungstate(VI)

Topics

• Animals
• Blood Glucose (drug effects)
• Body Weight (drug effects)
• Brain (drug effects)
• Diabetes Mellitus, Experimental
• Disease Models, Animal
• Eating (drug effects)
• Hypoglycemic Agents (pharmacology)
• Male
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Tungsten Compounds (pharmacology)