Molecular proteomics imaging of tumor interfaces by mass spectrometry.

Abstract

The specific molecular profiles of ovarian cancer interface zones (IZ), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Two-dimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interface-specific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDI-IMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors.

• Authors: Suki Kang, Hyo Sup Shim, Jong Sik Lee, Dong Su Kim, Hak Yong Kim, Seong Hyun Hong, Pan Soo Kim, Joo Heon Yoon, Nam Hoon Cho
• Journal: Journal of proteome research (J Proteome Res) Vol. 9 Issue 2 Pg. 1157-64 (Feb 05 2010) ISSN: 1535-3907 [Electronic] United States
• PMID: 19821573 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Electrophoresis, Gel, Two-Dimensional
• Female
• Humans
• Immunohistochemistry
• Microscopy, Fluorescence
• Ovarian Neoplasms (chemistry)
• Principal Component Analysis
• Proteomics
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)