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Sister chromatid exchanges and chromosomal aberrations in Chinese hamster ovary (CHO-K1) cells treated with the insecticide pirimicarb.

Abstract
Pirimicarb and its formulation Aficida (50% pirimicarb) effects were studied on CHO-K1 cells employing sister chromatid exchange (SCE), chromosomal aberrations (CA), cell-cycle progression and mitotic index analyses. Continuous treatments were performed within 10-300 microg/ml concentration-range. Pirimicarb, but not Aficida, induced a concentration-dependent increase of abnormal cells. Pirimicarb induced a greater frequency of chromatid/isochromatid breaks than Aficida did. Regression analyses showed a concentration-dependent increase in the frequency of chromatid-type breaks for both compounds whereas only the frequency of isochromatid-type breaks did in those pirimicarb-treated cultures. SCEs in pirimicarb- or Aficida-treated cultures were significantly higher than control values with concentrations of 100-200 microg/ml. Both test compounds induced equivalent frequency of SCEs. A delay in cell-cycle kinetics was observed for pirimicarb and Aficida within 100-300 and 200-300 microg/ml concentration-range, respectively. An inhibition of MI was observed for both chemicals regardless of tested concentrations. Finally, the CAs appears to be a higher sensitive bioassay to detect DNA damage at lower concentrations of pirimicarb than SCEs does. The results demonstrated that pirimicarb and Aficida exert geno-cytotoxicity, at least in CHO-K1 cells.
AuthorsSonia Soloneski, Marcelo L Larramendy
JournalJournal of hazardous materials (J Hazard Mater) Vol. 174 Issue 1-3 Pg. 410-5 (Feb 15 2010) ISSN: 1873-3336 [Electronic] Netherlands
PMID19811874 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Carbamates
  • Insecticides
  • Pyrimidines
  • pirimicarb
Topics
  • Animals
  • CHO Cells
  • Carbamates (toxicity)
  • Chromosome Aberrations
  • Cricetinae
  • Cricetulus
  • Dose-Response Relationship, Drug
  • Insecticides (toxicity)
  • Pyrimidines (toxicity)
  • Sister Chromatid Exchange

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