Abnormal cell-cycle expression of the proteins p27, mdm2 and cathepsin B in oral squamous-cell carcinoma infected with human papillomavirus.

Abstract

Since the role of human papillomavirus (HPV) infection in oral carcinogenesis is still unclear, the purpose of this study was to verify the association between the expression of p27, mdm2 and cathepsin B and by HPV-related oral lesions. Fifty-five oral biopsies were studied and HPV detection and typing (6/11, 16, 18, 31 and 33) were performed using polymerase chain reaction techniques. The distribution p27, mdm2 and cathepsin B was determined by immunohistochemistry. Twenty-one (38%) out of the 55 oral lesions tested positive for HPV, of which 6 (33%) were HPV 6/11, 1 (5%) was HPV 16, 14 (72%) were HPV 18 and none was HPV 33/31. Among the 55 biopsies, immunopositivity for p27, mdm2 and cathepsin B was observed in 17 (30.9%), 37 (67.2%) and 37 (67.2%), respectively. Among 21 HPV-positive oral lesions, immunopositivity of mdm2, p27 and cathepsin B was found, respectively, in 6 (33%) out of 18 benign lesions (BL), 4 (22%) out of 18 potential malignant epithelial lesions (PMEL) and 11 (57.9%) out of 19 malignant lesions (ML). High-risk HPV types may be associated with oral carcinoma, by cell-cycle control dysregulation, contributing to oral carcinogenesis and the overexpression of mdm2, p27 and cathepsin B.

• Authors: Renata Cristina Mazon, Thaís Rovigatti Gerbelli, Carlos Benatti Neto, Maria Rita Brancini de Oliveira, Eduardo Antonio Donadi, Maria Alice Guimarães Gonçalves, Edson Garcia Soares, Carla Patrícia Klay, Ivarne Tersariol, Maria Aparecida Pinhal, Luis Resende, Christiane Pienna Soares
• Journal: Acta histochemica (Acta Histochem) Vol. 113 Issue 2 Pg. 109-16 (Feb 2011) ISSN: 1618-0372 [Electronic] Germany
• PMID: 19811804 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2009 Elsevier GmbH. All rights reserved.

Chemical References

• Cyclin-Dependent Kinase Inhibitor p27
• Proto-Oncogene Proteins c-mdm2
• Cathepsin B

Topics

• Carcinoma, Squamous Cell (genetics, virology)
• Cathepsin B (analysis, biosynthesis, genetics)
• Cell Cycle (genetics)
• Cyclin-Dependent Kinase Inhibitor p27 (analysis, biosynthesis, genetics)
• Humans
• Immunohistochemistry
• Mouth Neoplasms (genetics, virology)
• Papillomavirus Infections (complications, genetics)
• Proto-Oncogene Proteins c-mdm2 (analysis, biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction