[Isolation and functional analysis of cotton universal stress protein promoter in response to phytohormones and abiotic stresses].

Abstract

The 949 bp promoter fragment upstream from the translation initiation site of the GUSP gene encoding a universal stress protein was isolated from the genomic DNA of Gossypium arboream. Some putative cis-acting elements involved in stress responses including E-box, ABRE, DPBF-box, and MYB-core elements were found in the promoter region. In an Agrobacterium-mediated transient expression assay, strong activation of the GUSP full promoter region occurred in tobacco leaves following dehydration, abscisic acid, salt, heavy metal, gibberellic acid and dark treatments. Deletion analysis of the promoter revealed that the dehydration, abscisic acid and salt responses were affected by the deletion between -208 and -949 bp and showed 2-4-fold induction. However, in response to dark, gibberellic acid and heavy metals the induction was only 2-fold. This is an important study as no report of this universal stress protein promoter is available in literature.

• Authors: Muzna Zahur, Asma Maqbool, Muhamad Ifran, Muhammad Younas Khan Barozai, Bushra Rashid, Sheikh Riazuddin, Tayyab Husnain
• Journal: Molekuliarnaia biologiia (Mol Biol (Mosk)) 2009 Jul-Aug Vol. 43 Issue 4 Pg. 628-35 ISSN: 0026-8984 [Print] Russia (Federation)
• PMID: 19807025 (Publication Type: English Abstract, Journal Article)

Chemical References

• Plant Growth Regulators
• Plant Proteins
• Transcription Factors

Topics

• Gossypium (genetics, metabolism)
• Plant Growth Regulators (genetics, metabolism)
• Plant Proteins (biosynthesis, genetics)
• Response Elements (genetics)
• Rhizobium (genetics, metabolism)
• Sequence Deletion
• Stress, Physiological (genetics)
• Transcription Factors (genetics, metabolism)