Abstract | INTRODUCTION: Cytotoxic chemotherapy induces or worsens hemostatic disorders in patients with acute myeloid leukemia. Procoagulant release and tissue factor expression on tumor cells are considered to contribute to chemotherapeutic agents-associated coagulopathy. The role of red blood cells during this process has not been determined; although they lack tissue factor, they may contribute suitable membranes for the amplification phase of blood coagulation. The present study aims to evaluate the possible impact of daunorubicin on phosphatidylserine exposure and consequent procoagulant property of red blood cells. MATERIALS AND METHODS: RESULTS:
Daunorubicin treatment increased procoagulant activity of red blood cells regardless of the cell origin. Moreover, coagulation complexes assays supported daunorubicin-evoked procoagulant response. The procoagulant property of red blood cells was not affected by the other three agents. The modulating effect of procoagulant activity was concomitant with and dependent on level of phosphatidylserine on the outer surface. Blockade of phosphatidylserine with lactadherin inhibited over 90% of tenase generation and prothrombinase activity and prolonged the coagulation time. CONCLUSIONS:
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Authors | Jin Zhou, Yining Zheng, Jialan Shi, Chengfang Lu, Jinxiao Hou, Hongjuan Yu, Xin Qiao, Sen Qi, Gary E Gilbert |
Journal | Thrombosis research
(Thromb Res)
Vol. 125
Issue 2
Pg. 178-83
(Feb 2010)
ISSN: 1879-2472 [Electronic] United States |
PMID | 19800661
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2009 Elsevier Ltd. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Phosphatidylserines
- Daunorubicin
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Topics |
- Antineoplastic Agents
(pharmacology)
- Blood Coagulation
(drug effects, physiology)
- Case-Control Studies
- Daunorubicin
(pharmacology)
- Dose-Response Relationship, Drug
- Erythrocytes
(drug effects, metabolism)
- Humans
- Leukemia, Myeloid, Acute
(metabolism)
- Phosphatidylserines
(metabolism)
- Time Factors
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