Abstract |
Haptoglobin (HP) is an acute phase protein synthesized by liver cells in response to IL-6. HP has been demonstrated to modulate the immune response and to have anti-inflammatory activities. To analyze HP's effect on autoimmune inflammation, we here studied the course of EAE induced by immunization of Hp knockout (Hp(-/-)) and syngeneic WT mice with myelin oligodendrocyte glycoprotein peptide (MOG(35-55)). Hp(-/-)mice suffered from a more severe disease that was associated with increased expression of IL-17A, IL-6, and IFN-gamma mRNA in the CNS and with a denser cellular infiltrate in the spinal cord. During the recovery phase, a significantly higher number of myeloid DC, CD8+ cells, IL-17+ CD4+ and IFN-gamma+ CD4+ cells persisted in the CNS of Hp(-/-) mice. Absence of HP affected the priming and differentiation of T cells after MOG(35-55) immunization, as levels of Th2 cytokines produced in response to MOG stimulation by Hp(-/-) T cells were reduced. These results suggest that HP plays a modulatory and protective role on autoimmune inflammation of the CNS.
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Authors | Georgina Galicia, Wim Maes, Bert Verbinnen, Ahmad Kasran, Dominique Bullens, Mohamed Arredouani, Jan L Ceuppens |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 39
Issue 12
Pg. 3404-12
(Dec 2009)
ISSN: 1521-4141 [Electronic] Germany |
PMID | 19795414
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glycoproteins
- Haptoglobins
- Immunoglobulin G
- Interleukin-17
- Mog protein, mouse
- Myelin Proteins
- Myelin-Associated Glycoprotein
- Myelin-Oligodendrocyte Glycoprotein
- Peptide Fragments
- Transforming Growth Factor beta
- myelin oligodendrocyte glycoprotein (35-55)
- Interleukin-10
- Interferon-gamma
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Topics |
- Animals
- Autoimmune Diseases
(genetics, immunology, metabolism)
- Brain
(immunology, metabolism, pathology)
- Encephalomyelitis, Autoimmune, Experimental
(genetics, immunology, metabolism)
- Enzyme-Linked Immunosorbent Assay
- Glycoproteins
(immunology)
- Haptoglobins
(deficiency, genetics, metabolism)
- Immunization
- Immunoglobulin G
(blood)
- Inflammation
(genetics, immunology, metabolism)
- Interferon-gamma
(genetics, metabolism)
- Interleukin-10
(genetics)
- Interleukin-17
(genetics)
- Lymph Nodes
(metabolism, pathology)
- Mice
- Mice, Inbred BALB C
- Mice, Knockout
- Myelin Proteins
- Myelin-Associated Glycoprotein
(chemistry, immunology)
- Myelin-Oligodendrocyte Glycoprotein
- Peptide Fragments
(immunology)
- Reverse Transcriptase Polymerase Chain Reaction
- Spinal Cord
(immunology, metabolism, pathology)
- Spleen
(metabolism, pathology)
- T-Lymphocytes
(immunology, metabolism, pathology)
- Th2 Cells
(metabolism)
- Transforming Growth Factor beta
(genetics)
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