Abstract | AIM: The purpose of this study was to asses the impact of haemostatic and platelet receptor gene polymorphisms as an inherited risk factor for premature onset of myocardial infarction (MI). METHODS: Polymorphisms of platelet receptors - GP Ia (807C>T, rs1126643), GP VI (13254T>C, rs1613662), GP IIIa (HPA-1, rs5918), PAR -1 (IVS -14A>T; rs168753), P2Y(12) (34C>T, rs6785930 and H1/H2 haplotype, rs2046934), and genetic variations of the gene coding for cyclooxygenase-1 (COX-1) ( -842A>G, rs10306114 and 50C>T, rs3842787) were investigated. Mutations in the genes coding for coagulation factor V (Q506R (Leiden) mutation, rs6025) and factor II ( prothrombin G20210A, rs1799963) were also determined. The prevalence of gene polymorphisms was investigated in 105 consecutive patients with premature MI. This was compared with the same gene polymorphism prevalence in a group of 132 patients in which coronary artery disease had been excluded. Genotyping was done using PCR, followed by melting curve analysis with specific fluorescent hybridization probes. RESULTS: A significant association between GP VI 13254C allele carriers and premature MI was found (p=0.025). No other differences in prevalence of the investigated polymorphisms between the compared patient populations reached statistical significance. In a logistic regression, which took other cardiovascular risk factors into account, the significance of the GP VI 13254C allele and vascular risk was suggested (OR 1.888, 95% C.I. 1.029 to 3.464, p=0.040). In a binary logistic regression the positive relationship between the GP VI genotype and female gender was observed (0R 3.676; 95% C.I. 1.159 to 11.628; p=0.027). The frequencies of GP VI and GP Ia gene polymorphisms were independent of one another (p=0.836). CONCLUSION: The presence of the GP VI 13254C allele is an independent predictor of premature MI.
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Authors | Zuzana Motovska, Jan Kvasnicka, Petr Widimsky, Robert Petr, Jaroslava Hajkova, Petra Bobcikova, Pavel Osmancik, Denisa Odvodyova, Stanislav Katina |
Journal | Thrombosis research
(Thromb Res)
Vol. 125
Issue 2
Pg. e61-4
(Feb 2010)
ISSN: 1879-2472 [Electronic] United States |
PMID | 19786296
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2009 Elsevier Ltd. All rights reserved. |
Chemical References |
- Platelet Membrane Glycoproteins
- platelet membrane glycoprotein VI
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Topics |
- Adult
- Alleles
- Female
- Gene Frequency
- Genotype
- Humans
- Logistic Models
- Male
- Middle Aged
- Myocardial Infarction
(genetics)
- Platelet Membrane Glycoproteins
(analysis, genetics)
- Polymorphism, Genetic
- Randomized Controlled Trials as Topic
- Risk Factors
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