Modelling of tumour--host coexistence In vitro in the presence of serine protease inhibitors.

Abstract	The activities of cell surface serine proteases are markedly enhanced in malignant tumours. Proteolytic degradation of the extracellular matrix and basal membrane of normal cells is an important event for tumour cell growth and invasion. Two well-known broad-spectrum inhibitors of serine protease, Foy-305 and Ono-3403, were evaluated for their ability to affect the growth rate and survival of MCF7 breast cancer cells co-cultured with MRC5 lung fibroblasts as feeder cells in the absence of serum. Flow cytometry and differential staining demonstrated that in the mixed culture, the rate of tumor growth was dependent upon the presence of the feeder MRC5 lung fibroblasts and could be obviated by the additional presence of the inhibitors of serine proteases.
Authors	Helga Engi, Nóra Gyémánt, Motohiro Ohkoshi, Leonard Amaral, Joseph Molnár
Journal	In vivo (Athens, Greece) (In Vivo) 2009 Sep-Oct Vol. 23 Issue 5 Pg. 711-5 ISSN: 0258-851X [Print] Greece
PMID	19779105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents Benzamidines Esters Guanidines Serine Proteinase Inhibitors ethyl N-allyl-N-(2-methyl-3-(4-(4-amidinophenoxycarbonyl)phenyl)propenoyl)aminoacetate methanesulfonate camostat Allylglycine Gabexate Serine Endopeptidases
Topics	Allylglycine (analogs & derivatives, pharmacology) Antineoplastic Agents (pharmacology) Benzamidines (pharmacology) Breast Neoplasms (drug therapy, enzymology, pathology) Cell Line, Tumor Cell Membrane (drug effects, enzymology) Cell Proliferation (drug effects) Cell Survival (drug effects) Coculture Techniques Drug Screening Assays, Antitumor Esters Female Fibroblasts (drug effects, enzymology, pathology) Flow Cytometry Gabexate (analogs & derivatives, pharmacology) Guanidines Humans Serine Endopeptidases (metabolism) Serine Proteinase Inhibitors (pharmacology)

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