HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Inhibitory effect of piperlonguminine/ dihydropiperlonguminine on the production of amyloid beta and APP in SK-N-SH cells.

Abstract
The study was undertaken to explore whether piperlonguminine/dihydropiperlonguminine could inhibit the production of amyloidbeta (Abeta) in human neuroblastoma cells (SK-N-SH) and to examine the underlying mechanism of this effect. Piperlonguminine/dihydropiperlonguminine components (1:0.8) were extracted from Futokadsura stem, and then used to treat SK-N-SH cells at three different concentrations: 3.13 microg/ml, 6.25 microg/ml and 12.50 microg/ml. Subsequently, the production of Abeta42 and Abeta40 were measured by Western blot analysis and enzyme linked immunosorbent assay (ELISA). On the other hand, the expressions of amyloid precursor protein (APP), Notch1 (Notch intracellular domain) and beta-site amyloid precursor protein cleavage enzyme (BACE-1) were also examined by Western blot assay. The activities of beta-secretase and gamma-secretase were detected at the same time. Furthermore, Abeta42 level was detected by immunocytochemistry staining. We demonstrated that the treatment of piperlonguminine/dihydropiperlonguminine could significantly decrease the levels of APP, Abeta42 and Abeta40 peptide in SK-N-SH cells, despite the fact that the activities of beta-secretase and gamma-secretase were not affected significantly. These data suggest that piperlonguminine/dihydropiperlonguminine components could significantly inhibit the level of APP, Abeta42 and Abeta40 peptide without affecting the activity of beta-secretase and gamma-secretase in SK-N-SH cells.
AuthorsHong-Shun Qi, Pei Liu, Su-Qin Gao, Zeng-Yan Diao, Ling-Ling Yang, Jun Xu, Xun Qu, En-Ji Han
JournalThe Chinese journal of physiology (Chin J Physiol) Vol. 52 Issue 3 Pg. 160-8 (Jun 30 2009) ISSN: 0304-4920 [Print] India
PMID19777802 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amyloid beta-Peptides
  • Amyloid beta-Protein Precursor
  • Dioxolanes
  • Drugs, Chinese Herbal
  • NOTCH1 protein, human
  • Receptor, Notch1
  • Amyloid Precursor Protein Secretases
  • Aspartic Acid Endopeptidases
  • BACE1 protein, human
  • piperlonguminine
Topics
  • Amyloid Precursor Protein Secretases (metabolism)
  • Amyloid beta-Peptides (metabolism)
  • Amyloid beta-Protein Precursor (metabolism)
  • Aspartic Acid Endopeptidases (metabolism)
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • Dioxolanes (pharmacology)
  • Drugs, Chinese Herbal (pharmacology)
  • Humans
  • Neuroblastoma (metabolism, pathology)
  • Receptor, Notch1 (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: