Abstract | OBJECTIVE: We studied the role of type 17 Th cells (TH17) in the pathogenesis of SLE. METHODS: We quantified the mRNA expression of IL-17, -23, -27 and retinoic-acid-related orphan receptor ( ROR)-gamma, the regulator for the development and function of TH17, in the urinary sediment of 23 subjects with active lupus nephritis, 25 subjects with a history of lupus nephritis in remission, 30 SLE patients with no history of renal involvement and 8 healthy subjects. RESULTS: All three groups of lupus patients had a higher urinary expression of TH17-related cytokines than the controls. However, urinary expression of IL-17 and -27 was found to be inversely correlated with the SLEDAI score (r = -0.252 and -0.258, respectively; P < 0.05 for both). For patients with active lupus nephritis, the histological activity index of kidney biopsy was also found to be inversely correlated with the urinary expression of ROR-gamma (r = -0.447; P = 0.032), IL-17 (r = -0.454; P = 0.029) and IL-23 (r = -0.455; P = 0.029). Urinary expression of IL-17, -23, -27 and ROR was also found to be inversely correlated with the urinary expression of IFN-gamma and T-bet, the key transcription factor of type 1 Th cells. After 6 months of treatment, urinary IL-27 expression rose significantly in patients with complete response (from 2.07 +/- 1.62 to 3.70 +/- 1.69; P = 0.028) but remained unchanged in those with partial or no response (from 2.60 +/- 1.87 to 2.52 +/- 1.94; P = 0.9). CONCLUSIONS: The urinary expression of TH17-related genes is increased in SLE patients. The degree of up-regulation, however, is inversely related to systemic and renal lupus activity, as well as urinary expression of TH1-related genes. Urinary expression of TH17-related genes increased again after successful immunosuppressive treatment of active disease. Our findings suggest a regulatory role of TH17-related cytokines in pathogenesis of lupus nephritis.
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Authors | Bonnie Ching-Ha Kwan, Lai-Shan Tam, Ka-Bik Lai, Fernand Mac-Moune Lai, Edmund Kwok-Ming Li, Gang Wang, Kai-Ming Chow, Philip Kam-Tao Li, Cheuk-Chun Szeto |
Journal | Rheumatology (Oxford, England)
(Rheumatology (Oxford))
Vol. 48
Issue 12
Pg. 1491-7
(Dec 2009)
ISSN: 1462-0332 [Electronic] England |
PMID | 19773408
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adult
- Cytokines
(genetics, urine)
- Female
- Follow-Up Studies
- Gene Expression Regulation
(immunology)
- Humans
- Lupus Erythematosus, Systemic
(immunology)
- Lupus Nephritis
(immunology)
- Male
- Middle Aged
- RNA, Messenger
(genetics)
- Severity of Illness Index
- T-Lymphocytes, Helper-Inducer
(immunology)
- Th1 Cells
(immunology)
- Th2 Cells
(immunology)
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