[Differences in transcription of nuclear RNA from rat liver with normal and malignant growth].

Abstract

Total nuclear RNA was isolated under conditions of actinomycin D blocking ion-exchange chromatography on kieselguhr columns with methylated albumin detected differences in transpiration of rat liver nuclear RNA with intensive normal and malignant growth. Actinomycin D in doses blocking the appearance of peculiar proteins in the blood serum of rats with the regenerating liver and RS-1 hepatoma produces a different effect on the biosynthesis of nuclear DNA-like RNA of the rat liver. 24h after a partial hepatectomy the antibiotic inhibits considerably the biosynthesis of nuclear DNA-like RNA which is eluated during chromatographying with 0.2% solution of sodium dodecyl sulphate at 70 degrees C. With RS-1 hepatoma the actinomycin D effect is most pronounced with respect to nuclear DNA-like RNA of rats with a tumour which is washed off from the column with a 0.2% solution of sodium dodecyl sulphate at 37 degrees C.

• Authors: A S Polishchuk, V P Korotkoruchko
• Journal: Ukrains'kyi biokhimichnyi zhurnal (Ukr Biokhim Zh) 1977 Jul-Aug Vol. 49 Issue 4 Pg. 80-4 ISSN: 0041-610X [Print] Ukraine
• Vernacular Title: Vidminnosti u transkriptsii iadernikh RNK pechinki shchuriv za normal'nogo i zloiakisnogo rostu.
• PMID: 197675 (Publication Type: English Abstract, Journal Article)

Chemical References

• Blood Proteins
• Dactinomycin
• RNA

Topics

• Animals
• Blood Proteins (metabolism)
• Carcinoma, Hepatocellular (metabolism)
• Cell Nucleus (metabolism)
• Dactinomycin (pharmacology)
• Hepatectomy
• Liver (drug effects, metabolism)
• Liver Neoplasms (metabolism)
• Liver Regeneration
• Neoplasms, Experimental (metabolism)
• RNA (biosynthesis)
• Rats
• Transcription, Genetic