Abstract |
We investigated whether systemically administered EP2 receptor agonists would stimulate angiogenesis in the emphysematous lungs of mice. Saline or porcine pancreatic elastase was intratracheally administered to C57BL/6J mice to induce the formation of emphysematous lesions, and 4 weeks later the mice were intraperitoneally injected with an EP2 receptor agonist, ONO-AE1-259 or PGE2, or saline on days 1-5 each week for 3 weeks. Intraperitoneal ONO-AE1-259 in the mice in the intratracheal saline group increased pulmonary capillary volume to 114.9% of the control value (P<0.05), and when administered to the intratracheal elastase group, ONO-AE1-259 partially restored pulmonary capillary volume, from 70.9% of the control value to 88.3% of the control value (P<0.05). Intraperitoneal PGE2 tended to increase pulmonary capillary volume in the mice in the intratracheal saline group (P=0.07) but not in the intratracheal elastase group. Intraperitoneal ONO-AE1-259 and PGE2 each increased the concentration of vascular endothelial growth factor ( VEGF) and the number of endothelial progenitor cells (EPCs) in circulating blood. These results suggest that systemically administered ONO-AE1-259 stimulates pulmonary angiogenesis in an elastase-induced murine model of emphysema.
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Authors | Takao Tsuji, Kazutetsu Aoshiba, Naoko Yokohori, Atsushi Nagai |
Journal | Prostaglandins & other lipid mediators
(Prostaglandins Other Lipid Mediat)
Vol. 90
Issue 3-4
Pg. 85-8
(Dec 2009)
ISSN: 1098-8823 [Print] United States |
PMID | 19765669
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 9-deoxy-9-chloro-15-deoxy-16-hydroxy-17,17-trimethylene-19,20-didehydroprostaglandin E2
- Ptger2 protein, mouse
- Receptors, Prostaglandin E
- Receptors, Prostaglandin E, EP2 Subtype
- Vascular Endothelial Growth Factor A
- Dinoprostone
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Topics |
- Animals
- Dinoprostone
(analogs & derivatives, pharmacology, physiology)
- Disease Models, Animal
- Lung
(blood supply, pathology, ultrastructure)
- Male
- Mice
- Mice, Inbred C57BL
- Microscopy, Electron, Transmission
- Neovascularization, Physiologic
(drug effects)
- Pulmonary Emphysema
(drug therapy, pathology)
- Receptors, Prostaglandin E
(agonists)
- Receptors, Prostaglandin E, EP2 Subtype
- Vascular Endothelial Growth Factor A
(blood)
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