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[Study on proteomics of Jurkat cells treated with the extracts from Prunella vulgaris].

AbstractOBJECTIVE:
To analyse the differential proteins of Jurkat cells after treated with the extracts from Prunella vulgaris using two-dimensional electrophoresis and mass spectrum.
METHODS:
Jurkat cell growth inhibitive effect of the extracts from Prunella vulgaris was analyzed by MTT assay. The total proteins of the cells were extracted after treated with the extracts in a dose of 20 microg/mL. Then 2D and MALDI-TOF-MS were used to assess the differential proteins.
RESULTS:
The extracts from Prunella vulgaris could depress the proliferation of Jurkat cells in a dose-dependent manner. After 2-DE and MALDI-TOF-MS,11 proteins were identified successfully, including glyceraldehyde-3-phosphate dehydrogenase, coagulation factor VII, Heterogeneous nuclear ribonucleoprotein L, heat shock 70 kDa protein 8 isoform 2, immunoglobulin heavy chain variable region, heterogeneous nuclear ribonucleoprotein A2/B1, zinc finger protein 43, chaperonin containing TCP1, subunit 6A (zeta 1), isoform CRA_b, etc.
CONCLUSION:
The extracts from Prunella vulgaris could inhibit the growth of Jurkat cells significantly, and lead the proteomics change of Jurkat cells, which may be related to the anti-tumor effect of the extracts from Prunella vulgaris.
AuthorsMing-Zhi Zhang, Zhen-Chang Sun, Xiao-Rui Fu, Chang-Ying Chen, Meng-Jie Ding
JournalZhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 32 Issue 6 Pg. 917-22 (Jun 2009) ISSN: 1001-4454 [Print] China
PMID19764330 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Drugs, Chinese Herbal
  • HSC70 Heat-Shock Proteins
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B
  • Kruppel-Like Transcription Factors
  • Repressor Proteins
  • ZNF43 protein, human
  • hnRNP A2
Topics
  • Antineoplastic Agents, Phytogenic (pharmacology)
  • Cell Proliferation (drug effects)
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Drugs, Chinese Herbal (pharmacology)
  • Electrophoresis, Gel, Two-Dimensional
  • HSC70 Heat-Shock Proteins (metabolism)
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B (metabolism)
  • Humans
  • Jurkat Cells
  • Kruppel-Like Transcription Factors (metabolism)
  • Proteomics
  • Prunella (chemistry)
  • Repressor Proteins (metabolism)
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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