Abstract | OBJECTIVE: To analyse the differential proteins of Jurkat cells after treated with the extracts from Prunella vulgaris using two-dimensional electrophoresis and mass spectrum. METHODS: Jurkat cell growth inhibitive effect of the extracts from Prunella vulgaris was analyzed by MTT assay. The total proteins of the cells were extracted after treated with the extracts in a dose of 20 microg/mL. Then 2D and MALDI-TOF-MS were used to assess the differential proteins. RESULTS: The extracts from Prunella vulgaris could depress the proliferation of Jurkat cells in a dose-dependent manner. After 2-DE and MALDI-TOF-MS,11 proteins were identified successfully, including glyceraldehyde-3-phosphate dehydrogenase, coagulation factor VII, Heterogeneous nuclear ribonucleoprotein L, heat shock 70 kDa protein 8 isoform 2, immunoglobulin heavy chain variable region, heterogeneous nuclear ribonucleoprotein A2/B1, zinc finger protein 43, chaperonin containing TCP1, subunit 6A (zeta 1), isoform CRA_b, etc. CONCLUSION: The extracts from Prunella vulgaris could inhibit the growth of Jurkat cells significantly, and lead the proteomics change of Jurkat cells, which may be related to the anti-tumor effect of the extracts from Prunella vulgaris.
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Authors | Ming-Zhi Zhang, Zhen-Chang Sun, Xiao-Rui Fu, Chang-Ying Chen, Meng-Jie Ding |
Journal | Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
(Zhong Yao Cai)
Vol. 32
Issue 6
Pg. 917-22
(Jun 2009)
ISSN: 1001-4454 [Print] China |
PMID | 19764330
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Drugs, Chinese Herbal
- HSC70 Heat-Shock Proteins
- Heterogeneous-Nuclear Ribonucleoprotein Group A-B
- Kruppel-Like Transcription Factors
- Repressor Proteins
- ZNF43 protein, human
- hnRNP A2
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Drug Screening Assays, Antitumor
- Drugs, Chinese Herbal
(pharmacology)
- Electrophoresis, Gel, Two-Dimensional
- HSC70 Heat-Shock Proteins
(metabolism)
- Heterogeneous-Nuclear Ribonucleoprotein Group A-B
(metabolism)
- Humans
- Jurkat Cells
- Kruppel-Like Transcription Factors
(metabolism)
- Proteomics
- Prunella
(chemistry)
- Repressor Proteins
(metabolism)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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