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Immunosuppressive effects of mesenchymal stem cells in collagen-induced mouse arthritis.

AbstractOBJECTIVE:
The objective of this study was to investigate the efficacy of mesenchymal stem cell (MSC) in the treatment of arthritis.
METHODS:
Mesenchymal stem cells were injected intravenously into mice with collagen-induced arthritis (CIA). Arthritic indexes were evaluated, and the levels of the pro- and anti-inflammatory cytokines interleukin-10 (IL-10), gamma interferon (IFN-gamma)-inducible protein 10 (IP-10), chemokine (C-X-C motif) receptor 3 (CXCR3), interleukin-17A (IL-17A), and tumor necrosis factor alpha (TNF-alpha) in serum or splenic cells were determined using real-time RT-PCR, Western blotting, and enzyme-linked immunosorbent assay (ELISA). The proliferation of dendritic cell line D2SC cells was determined using (3)H-TdR incorporation assay.
RESULTS:
Upon injection of MSCs, overall arthritis symptoms were significantly improved in the CIA mouse models as indicated by the paw edema. Consistent with this observation, serum levels of pro-inflammatory cytokine TNF-alpha and inflammatory cell infiltration decreased significantly 12 days after MSC injection, while the expression of anti-inflammatory cytokines IL-10, IP-10, and CXCR3 was increased in splenocytes. In addition, we provided evidence that MSCs may directly promote the proliferation of D2SC cells and the expression of IP-10 in D2SC cells in vitro.
CONCLUSION:
Mesenchymal stem cells significantly enhance the efficacy of collagen-induced arthritis treatment, likely through the modulation of the expression of various cytokines.
AuthorsFei Mao, Wen-Rong Xu, Hui Qian, Wei Zhu, Yong-Min Yan, Qi-Xiang Shao, Hua-Xi Xu
JournalInflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 59 Issue 3 Pg. 219-25 (Mar 2010) ISSN: 1420-908X [Electronic] Switzerland
PMID19763787 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chemokine CXCL10
  • Cytokines
Topics
  • Animals
  • Arthritis, Experimental (metabolism, physiopathology, therapy)
  • Cell Line
  • Cell Proliferation
  • Cell- and Tissue-Based Therapy (methods)
  • Chemokine CXCL10 (metabolism)
  • Cytokines (metabolism)
  • Disease Models, Animal
  • Male
  • Mesenchymal Stem Cells (physiology)
  • Mice
  • Mice, Inbred DBA

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