Abstract |
Herpes simplex virus type 1 (HSV-1) glycoprotein C (gC-1) binds complement component C3b and inhibits complement-mediated immunity. HSV-1 glycoprotein D (gD-1) is a potent immunogen and a candidate antigen for a subunit vaccine. We evaluated whether combined immunization with gD-1 and gC-1 provides better protection against challenge than gD-1 alone based on antibodies to gC-1 preventing HSV-1-mediated immune evasion. IgG purified from mice immunized with gC-1 blocked C3b binding to gC-1 and greatly increased neutralization by gD-1 IgG in the presence of complement. Passive transfer of gC-1 IgG protected complement intact mice against HSV-1 challenge but not C3 knockout mice, indicating that gC-1 antibody activity in vivo is complement-dependent. Immunizing mice with gD-1 and gC-1 provided better protection than gD-1 alone in preventing zosteriform disease and infection of dorsal root ganglia. Therefore, gC-1 immunization prevents HSV-1 evasion from complement and enhances the protection provided by gD-1 immunization.
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Authors | Sita Awasthi, John M Lubinski, Harvey M Friedman |
Journal | Vaccine
(Vaccine)
Vol. 27
Issue 49
Pg. 6845-53
(Nov 16 2009)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 19761834
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Herpes Simplex Virus Vaccines
- Immunoglobulin G
- Vaccines, Subunit
- Viral Envelope Proteins
- glycoprotein D, Human herpesvirus 1
- glycoprotein gC, herpes simplex virus type 1
- Complement C3b
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Topics |
- Animals
- Antibodies, Viral
(immunology)
- Chlorocebus aethiops
- Complement C3b
(immunology)
- Dose-Response Relationship, Immunologic
- Female
- Ganglia, Spinal
(immunology)
- Herpes Simplex
(immunology, prevention & control)
- Herpes Simplex Virus Vaccines
(immunology)
- Herpesvirus 1, Human
(immunology)
- Immune Evasion
- Immunization, Passive
- Immunoglobulin G
(immunology)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Knockout
- Neutralization Tests
- Vaccines, Subunit
(immunology)
- Vero Cells
- Viral Envelope Proteins
(immunology)
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