HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Immunization with HSV-1 glycoprotein C prevents immune evasion from complement and enhances the efficacy of an HSV-1 glycoprotein D subunit vaccine.

Abstract
Herpes simplex virus type 1 (HSV-1) glycoprotein C (gC-1) binds complement component C3b and inhibits complement-mediated immunity. HSV-1 glycoprotein D (gD-1) is a potent immunogen and a candidate antigen for a subunit vaccine. We evaluated whether combined immunization with gD-1 and gC-1 provides better protection against challenge than gD-1 alone based on antibodies to gC-1 preventing HSV-1-mediated immune evasion. IgG purified from mice immunized with gC-1 blocked C3b binding to gC-1 and greatly increased neutralization by gD-1 IgG in the presence of complement. Passive transfer of gC-1 IgG protected complement intact mice against HSV-1 challenge but not C3 knockout mice, indicating that gC-1 antibody activity in vivo is complement-dependent. Immunizing mice with gD-1 and gC-1 provided better protection than gD-1 alone in preventing zosteriform disease and infection of dorsal root ganglia. Therefore, gC-1 immunization prevents HSV-1 evasion from complement and enhances the protection provided by gD-1 immunization.
AuthorsSita Awasthi, John M Lubinski, Harvey M Friedman
JournalVaccine (Vaccine) Vol. 27 Issue 49 Pg. 6845-53 (Nov 16 2009) ISSN: 1873-2518 [Electronic] Netherlands
PMID19761834 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Herpes Simplex Virus Vaccines
  • Immunoglobulin G
  • Vaccines, Subunit
  • Viral Envelope Proteins
  • glycoprotein D, Human herpesvirus 1
  • glycoprotein gC, herpes simplex virus type 1
  • Complement C3b
Topics
  • Animals
  • Antibodies, Viral (immunology)
  • Cercopithecus aethiops
  • Complement C3b (immunology)
  • Dose-Response Relationship, Immunologic
  • Female
  • Ganglia, Spinal (immunology)
  • Herpes Simplex (immunology, prevention & control)
  • Herpes Simplex Virus Vaccines (immunology)
  • Herpesvirus 1, Human (immunology)
  • Immune Evasion
  • Immunization, Passive
  • Immunoglobulin G (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neutralization Tests
  • Vaccines, Subunit (immunology)
  • Vero Cells
  • Viral Envelope Proteins (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: