Abstract | OBJECTIVE: To observe the expression of plasma thrombospondin-1(TSP-1) at different time in protein-overload rats and to analyze the relationship between plasma TSP-1 expression and renal interstitial fibrosis. METHODS: Forty-five male Sprague-Dawley rats were randomly divided into a bovine serum albumin (BSA) group and a control group after uninephrectomization. Rats with protein overload nephropathy induced by intraperitoneally injected BSA were used as a model (control group received saline). At the 1st, 5th, and 9th weekend, the level of 24 h proteinuria and renal function was assessed. Pathological changes were observed by electron and fluorescent microscopy. The expression of plasma TSP-1 was detected by Western blot. The relationship between plasma TSP-1 and tubulointerstitial lesions (TIL) score was analyzed. RESULTS: Twenty-four hour proteinuria and blood urea nitrogen (BUN) significantly increased in protein-overload rats compared with those in the control group. While protein-overload rats developed more severe fibrosis in the tubular and interstitium. Glomerulosclerosis index and TIL score were upregulated compared with those in the control group. The expression of TSP-1 increased significantly at the 5th and 9th weekend. The expression of TSP-1 was positively correlated with TIL score (r=0.836, P<0.01). CONCLUSION:
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Authors | Yuncheng Xia, Jing Nie, Zhilan Li, Lin Sun, Yinhong Liu, Guanghui Ling, Youmin Peng, Fuyou Liu |
Journal | Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
(Zhong Nan Da Xue Xue Bao Yi Xue Ban)
Vol. 34
Issue 8
Pg. 796-802
(Aug 2009)
ISSN: 1672-7347 [Print] China |
PMID | 19734591
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Fibrosis
(metabolism, pathology)
- Glomerulosclerosis, Focal Segmental
(pathology)
- Kidney
(metabolism, pathology)
- Male
- Nephrectomy
- Nephritis, Interstitial
(etiology, metabolism, pathology)
- Rats
- Rats, Sprague-Dawley
- Thrombospondin 1
(blood)
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