The antiepileptic drug topiramate is a substrate for human P-glycoprotein but not multidrug resistance proteins.

Abstract

PURPOSE:

Resistance to antiepileptic drugs (AEDs) is the major problem in the treatment of epilepsy. One of the candidate mechanisms of pharmacoresistance is the limitation of AED access to the seizure focus by overexpression of efflux transporters, including P-glycoprotein (Pgp) and multidrug resistance proteins (MRPs).In this respect, it is important to know which AEDs are substrates for such drug transporters in humans.

METHODS:

In the present study, we used polarized kidney cell lines (LLC, MDCK) transfected with human drug transporters (Pgp, MRP1, MRP2 or MRP5) to evaluate whether the AED topiramate is a substrate for any of these transporters. Known Pgp and MRP substrates were used for comparison.

RESULTS:

Basolateral-to-apical transport of topiramate, which could be counteracted with the Pgp inhibitor, tariquidar, was determined in Pgp overexpressing LLC cells, whereas topiramate was not transported by any of the MRPs. A comparison with previous experiments in the same transport assay showed that topiramate exhibited the most pronounced Pgp-mediated efflux transport among the AEDS that have been studied as yet.

CONCLUSIONS:

Thus, these data indicate that brain levels of topiramate may be affected by overexpression of Pgp as determined in patients with intractable epilepsy.

• Authors: Carlos Luna-Tortós, Bernhard Rambeck, Uwe H Jürgens, Wolfgang Löscher
• Journal: Pharmaceutical research (Pharm Res) Vol. 26 Issue 11 Pg. 2464-70 (Nov 2009) ISSN: 1573-904X [Electronic] United States
• PMID: 19730994 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Anticonvulsants
• Multidrug Resistance-Associated Proteins
• Recombinant Proteins
• Topiramate
• Fructose

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (metabolism)
• Animals
• Anticonvulsants (metabolism)
• Cell Line
• Epilepsy (drug therapy)
• Fructose (analogs & derivatives, metabolism)
• Humans
• Multidrug Resistance-Associated Proteins (metabolism)
• Recombinant Proteins (genetics, metabolism)
• Swine
• Topiramate