BAL fluid 8-isoprostane concentrations in eosinophilic bronchitis and asthma.

Abstract

BACKGROUND:

Oxidative stress has an important role in the pathophysiology of asthma. But oxidative stress of airway has not been assessed in patients with nonasthmatic eosinophilic bronchitis (EB). 8-epi-prostaglandin F2alpha (8-isoprostane) is a biomarker of oxidative stress.

OBJECTIVES:

We sought to determine whether oxidative stress (measured by 8-isoprostane) occurs in EB and whether 8-isoprostane is associated with airway function in EB and asthma.

METHODS:

We measured 8-isoprostane concentrations in the bronchoalveolar lavage (BAL) fluid from 11 subjects with EB, 10 subjects with asthma, and 9 healthy control subjects. 8-isoprostane was measured by enzyme immunoassays.

RESULTS:

We found that BAL fluid 8-isoprostane concentrations were raised both in EB and asthma. The median concentrations of 8-isoprostane in BAL fluid were significantly higher in subjects with asthma (12.78 pg/mL) when compared with EB (8.34 pg/mL) and healthy control subjects (5.07 pg/mL).

CONCLUSIONS:

Our study shows that oxidative stress is increased significantly in asthmatic subjects and the degree of oxidative stress in EB subjects is milder than that in asthma, as reflected by 8-isoprostane concentrations in the BAL fluid. The difference in airway function observed in subjects with EB and asthma could be associated with different elevation in 8-isoprostane concentration in the airways.

• Authors: Jiaxing Xie, Qingling Zhang, Nanshan Zhong, Kefang Lai
• Journal: The Journal of asthma : official journal of the Association for the Care of Asthma (J Asthma) Vol. 46 Issue 7 Pg. 712-5 (Sep 2009) ISSN: 1532-4303 [Electronic] England
• PMID: 19728211 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 8-epi-prostaglandin F2alpha
• Dinoprost

Topics

• Adult
• Asthma (metabolism, pathology, physiopathology)
• Bronchitis (metabolism, pathology, physiopathology)
• Bronchoalveolar Lavage Fluid (chemistry, cytology)
• Dinoprost (analogs & derivatives, analysis, metabolism)
• Eosinophilia (metabolism, pathology, physiopathology)
• Eosinophils (pathology)
• Female
• Forced Expiratory Volume (physiology)
• Humans
• Male
• Middle Aged
• Neutrophils (pathology)
• Oxidative Stress (physiology)
• Sputum (cytology)