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Efficient delivery of antisense oligodeoxyribonucleotide g3139 by human serum albumin-coated liposomes.

Abstract
Human serum albumin (HSA)-coated liposomal formulations were synthesized and evaluated for the delivery of antisense oligodeoxyribonucleotide (ODN) G3139 in KB human oral carcinoma cells. Liposomes composed of dimethyldioctadecyl ammonium bromide/egg phosphatidylcholine/alpha-tocopheryl polyethylene glycol 1000 succinate (58:40:2 molar ratio) complexed with G3139 and coated with HSA were investigated for Bcl-2 downregulating activity. Cellular uptake of HSA-coated liposome-ODN complexes was more efficient than the uncoated liposome-ODN complexes. Treatment of the cells with HSA-coated liposome-ODN complexes resulted in efficient Bcl-2 mRNA downregulation that was approximately 3-fold greater than with uncoated liposomes (p < 0.05) and 6-fold greater than with free ODN. The transfection efficiency of liposome-ODN complexes coated with HSA was dependent on the concentration of HSA used and on the contents of alpha-helix and beta-strand in HSA. HSA-coated liposomes are effective delivery vehicles for antisense ODN.
AuthorsWanlop Weecharangsan, Bo Yu, Yu Zheng, Shujun Liu, Jiu Xia Pang, L James Lee, Guido Marcucci, Robert J Lee
JournalMolecular pharmaceutics (Mol Pharm) 2009 Nov-Dec Vol. 6 Issue 6 Pg. 1848-55 ISSN: 1543-8392 [Electronic] United States
PMID19725564 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Liposomes
  • Proto-Oncogene Proteins c-bcl-2
  • Serum Albumin
  • Thionucleotides
  • oblimersen
Topics
  • Blotting, Western
  • Cell Line, Tumor
  • Circular Dichroism
  • Electrophoresis, Agar Gel
  • Humans
  • Liposomes (administration & dosage, chemistry)
  • Proto-Oncogene Proteins c-bcl-2 (antagonists & inhibitors, genetics, metabolism)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serum Albumin (chemistry)
  • Thionucleotides (administration & dosage)

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