Antiasthmatic activity of luteolin-7-O-glucoside from Ailanthus altissima through the downregulation of T helper 2 cytokine expression and inhibition of prostaglandin E2 production in an ovalbumin-induced asthma model.

Abstract

Previously, we reported that an ethanol extract of Ailanthus altissima has antiinflammatory activity in an ovalbumin (OVA)-sensitized murine asthmatic model. To determine the biological compounds from this plant, luteolin-7-O-glucoside (L7G) was isolated and its antiasthmatic activity was evaluated in an in vivo murine asthmatic model. L7G (10 to 100 mg/kg, per os (p.o.)) reduced the amount of eosinophil infiltration in bronchoalveolar lavage (BAL) fluid in a dose-dependent manner. In comparison, dexamethasone (5 mg/kg, p.o.), which was used as a positive control, also strongly inhibited the number of infiltrating eosinophils. L7G inhibited both the prostaglandin E(2) (PGE(2)) and serum immunoglobulin E level in BAL fluid in a dose-dependent manner. In addition, L7G inhibited the transcript profiles of interleukin (IL)-4, IL-5, and IL-13 mRNA expression levels in the murine asthma model, as determined using reverse transcription-polymerase chain reaction (RT-PCR). These results suggest that the antiasthmatic activity of L7G in OVA-induced lung inflammation may occur in part via the downregulation of T helper 2 cytokine transcripts as well as the inhibition of PGE(2) production.

• Authors: Meihua Jin, Ju Hye Yang, Eunkyung Lee, Yue Lu, Soonyoul Kwon, Kun Ho Son, Jong Keun Son, Hyeun Wook Chang
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 32 Issue 9 Pg. 1500-3 (Sep 2009) ISSN: 1347-5215 [Electronic] Japan
• PMID: 19721222 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Asthmatic Agents
• Anti-Inflammatory Agents, Non-Steroidal
• Cytokines
• Glucosides
• Ovalbumin
• luteolin-7-glucoside
• Dinoprostone
• Luteolin

Topics

• Ailanthus (immunology)
• Animals
• Anti-Asthmatic Agents (therapeutic use)
• Anti-Inflammatory Agents, Non-Steroidal (therapeutic use)
• Asthma (drug therapy, immunology, pathology)
• Cytokines (antagonists & inhibitors, biosynthesis)
• Dinoprostone (antagonists & inhibitors, biosynthesis)
• Disease Models, Animal
• Down-Regulation (drug effects, immunology)
• Female
• Glucosides (isolation & purification, therapeutic use)
• Luteolin (isolation & purification, therapeutic use)
• Mice
• Mice, Inbred BALB C
• Ovalbumin (administration & dosage, toxicity)
• Th2 Cells (drug effects, immunology, metabolism)