Abstract | BACKGROUND: METHODS: Routine WBs from 2 independent laboratories and ELISA were used to detect anti-MAG IgM in serum from 207 patients with neuropathy and controls. The sensitivity and specificity of these assays were compared and related to the patient clinical and electrophysiologic characteristics. RESULTS: In ELISA, anti-MAG antibodies were found in serum from 49 (72%) of 68 patients with demyelinating polyneuropathy and IgM monoclonal gammopathy. However, in this subgroup of patients, only 30 (44%) and 37 (54%) were positive in the 2 WBs. All of the patients positive in the 2 WBs were also positive in ELISA. A high correlation was found for IgM activity in ELISA to MAG and sulfate-3-glucuronyl paragloboside ( SGPG) (Spearman rho = 0.72, p < 0.0001), supporting the notion that the shared sulfated glucuronic acid moiety of MAG and SGPG is preserved. Most patients positive in anti-MAG ELISA had a slowly progressive sensory-motor demyelinating polyneuropathy, even if the WB was negative. In control groups, however, 4 WB-negative patients with a nondemyelinating monoclonal gammopathy-related polyneuropathy were positive in anti-MAG ELISA. The remaining samples were negative in ELISA. CONCLUSION:
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Authors | M L Kuijf, M Eurelings, A P Tio-Gillen, P A van Doorn, L H van den Berg, H Hooijkaas, J Stork, N C Notermans, B C Jacobs |
Journal | Neurology
(Neurology)
Vol. 73
Issue 9
Pg. 688-95
(Sep 01 2009)
ISSN: 1526-632X [Electronic] United States |
PMID | 19720975
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Biomarkers
- Globosides
- Immunoglobulin M
- Myelin-Associated Glycoprotein
- sulfate-3-glucuronyl paragloboside
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Topics |
- Autoantibodies
(analysis, blood)
- Biomarkers
(analysis, blood)
- Disease Progression
- Enzyme-Linked Immunosorbent Assay
(methods)
- Globosides
(analysis, blood)
- Humans
- Immunoglobulin M
(immunology)
- Monoclonal Gammopathy of Undetermined Significance
(complications, immunology, physiopathology)
- Myelin Sheath
(immunology, pathology)
- Myelin-Associated Glycoprotein
(immunology)
- Nerve Fibers, Myelinated
(immunology, pathology)
- Peripheral Nerves
(immunology, pathology, physiopathology)
- Polyneuropathies
(blood, immunology, physiopathology)
- Polyradiculoneuropathy
(blood, immunology, physiopathology)
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