Effect of the nonpeptide thrombopoietin receptor agonist Eltrombopag on bone marrow cells from patients with acute myeloid leukemia and myelodysplastic syndrome.

Abstract

Thrombocytopenia is a frequent symptom and clinical challenge in patients with myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Eltrombopag is a small molecule thrombopoietin receptor agonist that might be a new option to treat thrombocytopenia in these diseases, provided that it does not stimulate malignant hematopoiesis. In this work, we studied the effects of Eltrombopag on proliferation, apoptosis, differentiation, colony formation, and malignant self-renewal of bone marrow mononuclear cells of patients with AML and MDS. Malignant bone marrow mononuclear cells did not show increased proliferation, or increased clonogenic capacity at concentrations of Eltrombopag ranging from 0.1 to 30 microg/mL. On the contrary, we observed a moderate, statistically nonsignificant (P = .18), decrease of numbers of malignant cells (mean, 56%; SD, 28%). Eltrombopag neither led to increased 5-bromo-2-deoxyuridine incorporation, decreased apoptosis, an increase of malignant self-renewal, nor enhanced in vivo engraftment in xenotransplantations. Furthermore, we found that Eltrombopag was capable of increasing megakaryocytic differentiation and formation of normal megakaryocytic colonies in patients with AML and MDS. These results provide a preclinical rationale for further testing of Eltrombopag for treatment of thrombocytopenia in AML and MDS.

• Authors: Britta Will, Masahiro Kawahara, Julia P Luciano, Ingmar Bruns, Samir Parekh, Connie L Erickson-Miller, Manuel A Aivado, Amit Verma, Ulrich Steidl
• Journal: Blood (Blood) Vol. 114 Issue 18 Pg. 3899-908 (Oct 29 2009) ISSN: 1528-0020 [Electronic] United States
• PMID: 19710504 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Benzoates
• Hydrazines
• Pyrazoles
• Receptors, Thrombopoietin
• eltrombopag

Topics

• Animals
• Apoptosis (drug effects)
• Benzoates (pharmacology, therapeutic use)
• Bone Marrow Cells (metabolism, pathology)
• Cell Differentiation
• Cell Proliferation (drug effects)
• Dose-Response Relationship, Drug
• Hematopoiesis (drug effects)
• Humans
• Hydrazines (pharmacology, therapeutic use)
• Leukemia, Myeloid, Acute (drug therapy, metabolism, pathology)
• Leukocytes, Mononuclear (metabolism, pathology)
• Male
• Megakaryocytes (metabolism, pathology)
• Mice
• Mice, Inbred NOD
• Myelodysplastic Syndromes (drug therapy, metabolism, pathology)
• Pyrazoles (pharmacology, therapeutic use)
• Receptors, Thrombopoietin (agonists, metabolism)
• Thrombocytopenia (drug therapy, metabolism, pathology)
• Xenograft Model Antitumor Assays