TNF receptor-associated factor 1 is a positive regulator of the NF-kappaB alternative pathway.

Abstract

Tumor necrosis factor receptor-associated factor 1 (TRAF1) is unique among the members of the TRAF family, as it lacks the N-terminal RING/zinc-finger domain. Also the function of TRAF1 is not clearly established, with many papers reporting contradictory results. Here we show that TRAF1 interacts with BAFF receptor, a member of the TNF receptor family, and positively regulates activation of the alternative NF-kappaB pathway. Ectopic expression of TRAF1 causes degradation of TRAF3, stabilization of NIK, and processing of p100 to produce the mature form p52. In addition, we show that knocking-down expression of TRAF1 in the Hodgkin's disease derived cell line L1236, interfere with p100 processing and with p52 mediate gene transcription. Collectively these results support a role for TRAF1 as a positive regulator of the NF-kappaB alternative pathway.

• Authors: Alfonso Lavorgna, Rosaria De Filippi, Silvestro Formisano, Antonio Leonardi
• Journal: Molecular immunology (Mol Immunol) Vol. 46 Issue 16 Pg. 3278-82 (Oct 2009) ISSN: 1872-9142 [Electronic] England
• PMID: 19698991 (Publication Type: Journal Article)

Chemical References

• B-Cell Activation Factor Receptor
• NF-kappa B
• TNF Receptor-Associated Factor 1
• TNF Receptor-Associated Factor 3
• TNFRSF13C protein, human
• TRAF3 protein, human
• Protein Serine-Threonine Kinases
• NF-kappa B kinase

Topics

• B-Cell Activation Factor Receptor (biosynthesis, genetics, immunology)
• Cell Line, Tumor
• Gene Expression
• Hodgkin Disease (genetics, immunology, metabolism)
• Humans
• NF-kappa B (genetics, immunology, metabolism)
• Protein Serine-Threonine Kinases (genetics, immunology, metabolism)
• Protein Stability
• TNF Receptor-Associated Factor 1 (biosynthesis, genetics, immunology)
• TNF Receptor-Associated Factor 3 (genetics, immunology, metabolism)
• Zinc Fingers (genetics, immunology)