Novel screening method for agents that overcome classical multidrug resistance in a human cell line.

Abstract

P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrug-resistance (MDR) increased the accumulation of RG in a MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

• Authors: A Yoshimura, N Shudo, S I Ikeda, M Ichikawa, T Sumizawa, S I Akiyama
• Journal: Cancer letters (Cancer Lett) Vol. 50 Issue 1 Pg. 45-51 (Apr 09 1990) ISSN: 0304-3835 [Print] Ireland
• PMID: 1969768 (Publication Type: Journal Article)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Coloring Agents
• Membrane Glycoproteins
• Rhodamines
• Xanthenes
• rhodamine 6G
• Vincristine
• Verapamil

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Coloring Agents
• Drug Resistance
• Drug Screening Assays, Antitumor
• Humans
• KB Cells (drug effects)
• Membrane Glycoproteins (physiology)
• Rhodamines (pharmacokinetics)
• Verapamil (pharmacology)
• Vincristine (pharmacology)
• Xanthenes