Molecular and genetic characterization of human cell lines resistant to L-asparaginase and albizziin.

Abstract

Human cell lines resistant to L-asparaginase or albizziin were isolated by multistep selection of HT1080 fibrosarcoma and MIA PaCa-2 pancreatic carcinoma cells. Mutants were cross-resistant to both drugs, but more resistant to the drug used for selection. The drug-resistant cell lines expressed elevated levels of asparagine synthetase activity and protein, up to 17-fold over that of the parental cells. Enzyme overproduction was due to gene amplification in the albizziin-resistant cells, whereas increased expression without amplification was observed in L-asparaginase-resistant cells.

• Authors: I L Andrulis, R Argonza, A E Cairney
• Journal: Somatic cell and molecular genetics (Somat Cell Mol Genet) Vol. 16 Issue 1 Pg. 59-65 (Jan 1990) ISSN: 0740-7750 [Print] United States
• PMID: 1968681 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids, Diamino
• RNA, Messenger
• Asparaginase
• Ligases
• Aspartate-Ammonia Ligase
• albizziin

Topics

• Amino Acids, Diamino (pharmacology)
• Asparaginase (pharmacology)
• Aspartate-Ammonia Ligase (biosynthesis, genetics)
• Cell Line
• Drug Resistance (genetics)
• Gene Amplification
• Humans
• Ligases (genetics)
• RNA, Messenger (biosynthesis)
• Tumor Cells, Cultured