Preparation, characterization, and antiproliferative activities of the Se-containing polysaccharide SeGLP-2B-1 from Se-enriched Ganoderma lucidum.

Abstract

Inorganic selenite can be transformed into organic forms and bind to proteins and polysaccharides in Se-enriched submerged Ganoderma lucidum cultures. In the present study, a novel Se-containing polysaccharide, SeGLP-2B-1, was purified from the Se-enriched mycelia of G. lucidum and the antiproliferative activities against six human cancer cell lines were investigated. The Se content of SeGLP-2B-1 was 186.7 microg/g, which was 150-fold larger than that of the regular polysaccharide GLP-2B-1 (1.3 microg/g). SeGLP-2B-1 (1.06 x 10(6) Da) was composed of glucose, rhamnose, xylose, and galactose with a molar ratio of 1.000:0.652:0.443:0.227. SeGLP-2B-1 exhibited an approximately 10-fold stronger antiproliferative activity against six human cancer cell lines as compared to GLP-2B-1. Thus, Se is believed to play an important role in increasing the antiproliferative property of SeGLP-2B-1. These findings indicate that SeGLP-2B-1 may serve as a dietary Se supplement.

• Authors: Dejing Shang, Jianing Zhang, Lei Wen, Yang Li, Qiao Cui
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 57 Issue 17 Pg. 7737-42 (Sep 09 2009) ISSN: 1520-5118 [Electronic] United States
• PMID: 19678686 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Organometallic Compounds
• Polysaccharides
• SeGLP-2B-1
• Selenium

Topics

• Antineoplastic Agents (pharmacology)
• Breast Neoplasms
• Carcinoma, Hepatocellular
• Cell Cycle (drug effects)
• Cell Division (drug effects)
• Cell Line, Tumor
• Chemical Phenomena
• Dietary Supplements
• Female
• Ganoderma (chemistry)
• HeLa Cells
• Humans
• K562 Cells
• Liver Neoplasms
• Mycelium (chemistry)
• Organometallic Compounds (chemistry, isolation & purification, pharmacology)
• Polysaccharides (analysis, chemistry, isolation & purification, pharmacology)
• Selenium (administration & dosage, analysis)