Abstract |
Abscisic acid (ABA) is a plant hormone regulating fundamental physiological functions in plants, such as response to abiotic stress. Recently, ABA was shown to be produced and released by human granulocytes, by insulin-producing rat insulinoma cells, and by human and murine pancreatic beta cells. ABA autocrinally stimulates the functional activities specific for each cell type through a receptor-operated signal transduction pathway, sequentially involving a pertussis toxin-sensitive receptor/ G-protein complex, cAMP, CD38-produced cADP-ribose and intracellular calcium. Here we show that the lanthionine synthetase C-like protein LANCL2 is required for ABA binding on the membrane of human granulocytes and that LANCL2 is necessary for transduction of the ABA signal into the cell-specific functional responses in granulocytes and in rat insulinoma cells. Co-expression of LANCL2 and CD38 in the human HeLa cell line reproduces the ABA-signaling pathway. Results obtained with granulocytes and CD38(+)/LANCL2(+) HeLa transfected with a chimeric G-protein (G alpha(q/i)) suggest that the pertussis toxin-sensitive G-protein coupled to LANCL2 is a G(i). Identification of LANCL2 as a critical component of the ABA-sensing protein complex will enable the screening of synthetic ABA antagonists as prospective new anti-inflammatory and anti-diabetic agents.
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Authors | Laura Sturla, Chiara Fresia, Lucrezia Guida, Santina Bruzzone, Sonia Scarfì, Cesare Usai, Floriana Fruscione, Mirko Magnone, Enrico Millo, Giovanna Basile, Alessia Grozio, Emanuela Jacchetti, Marcello Allegretti, Antonio De Flora, Elena Zocchi |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 284
Issue 41
Pg. 28045-28057
(Oct 09 2009)
ISSN: 1083-351X [Electronic] United States |
PMID | 19667068
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- LANCL2 protein, human
- LANCL2 protein, rat
- Membrane Proteins
- Nuclear Proteins
- Phosphate-Binding Proteins
- RNA, Small Interfering
- Reactive Oxygen Species
- Abscisic Acid
- GDP-ribosyl cyclase
- N-Glycosyl Hydrolases
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Topics |
- Abscisic Acid
(metabolism)
- Animals
- Cell Membrane
(metabolism)
- Cell Movement
- Cells, Cultured
(metabolism)
- Granulocytes
(cytology, metabolism)
- Humans
- Insulinoma
(genetics, metabolism)
- Membrane Proteins
(genetics, metabolism)
- N-Glycosyl Hydrolases
(genetics, metabolism)
- Nuclear Proteins
(genetics, metabolism)
- Phagocytosis
(physiology)
- Phosphate-Binding Proteins
- Protein Binding
- RNA, Small Interfering
(genetics, metabolism)
- Rats
- Reactive Oxygen Species
(metabolism)
- Signal Transduction
(physiology)
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