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[Influce of lysophosphatidic acid on proliferation, adhesion, migration and apoptosis of cervical cancer HeLa cells].

AbstractAIM:
To observe the effect of lysophosphatidic acid on cervical cancer cell line HeLa cells proliferation, adhesion, migration and apoptosis and study related signaling pathway.
METHODS:
After HeLa cells affected by LPA, the level of proliferation was drawing growth curve after cell couting; the sensitivity to cisplatin was evaluated by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry was used to examine cell apoptosis; the potentiality of migration was evaluated by transwell chamber, then to observe the effect of Y27632, LY294002 and PD98059 on biological function of LPA.
RESULTS:
LPA could promote HeLa cell proliferate on time-dependent and dose-dependent relationship. MEK1 inhibitor could inhibit LPA-induced cell proliferation; LPA promoted the adhesion and migration of HeLa cells in dose-dependence; Rho inhibitor could inhibit the LPA-induced adhesion and migration; LPA could reduce the chemotherapy sensibility of cervical cancer to DDP in dose-dependence, then PI3K inhibitor could resist this effect.
CONCLUSION:
LPA could promote HeLa cells' proliferation, adhesion, migration and anti-apoptosis through multiple signaling pathways.
AuthorsHong Sun, Qing Zhu, Juan Ren, Lei Wu, Fang-zhong Kong, Guang Li, Hai-feng Hu
JournalXi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 25 Issue 8 Pg. 702-5 (Aug 2009) ISSN: 1007-8738 [Print] China
PMID19664393 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Lysophospholipids
  • lysophosphatidic acid
Topics
  • Apoptosis
  • Cell Adhesion
  • Cell Proliferation
  • Female
  • HeLa Cells
  • Humans
  • Lysophospholipids (metabolism)
  • Uterine Cervical Neoplasms (metabolism, physiopathology)

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