Abstract | AIM: METHODS: After HeLa cells affected by LPA, the level of proliferation was drawing growth curve after cell couting; the sensitivity to cisplatin was evaluated by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry was used to examine cell apoptosis; the potentiality of migration was evaluated by transwell chamber, then to observe the effect of Y27632, LY294002 and PD98059 on biological function of LPA. RESULTS: LPA could promote HeLa cell proliferate on time-dependent and dose-dependent relationship. MEK1 inhibitor could inhibit LPA-induced cell proliferation; LPA promoted the adhesion and migration of HeLa cells in dose-dependence; Rho inhibitor could inhibit the LPA-induced adhesion and migration; LPA could reduce the chemotherapy sensibility of cervical cancer to DDP in dose-dependence, then PI3K inhibitor could resist this effect. CONCLUSION: LPA could promote HeLa cells' proliferation, adhesion, migration and anti-apoptosis through multiple signaling pathways.
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Authors | Hong Sun, Qing Zhu, Juan Ren, Lei Wu, Fang-zhong Kong, Guang Li, Hai-feng Hu |
Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
(Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi)
Vol. 25
Issue 8
Pg. 702-5
(Aug 2009)
ISSN: 1007-8738 [Print] China |
PMID | 19664393
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lysophospholipids
- lysophosphatidic acid
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Topics |
- Apoptosis
- Cell Adhesion
- Cell Proliferation
- Female
- HeLa Cells
- Humans
- Lysophospholipids
(metabolism)
- Uterine Cervical Neoplasms
(metabolism, physiopathology)
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