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Use of monoclonal antibody against major internal protein p24 of bovine leukemia virus in capture ELISA.

Abstract
A monoclonal antibody (4H4) against the major internal protein, p24, of bovine leukemia virus (BLV) is described. It recognizes a sequence determinant on the p24-molecule and displays high affinity to its antigen. The monoclonal antibody 4H4 was applied in capture ELISA for diagnosis of enzootic bovine leukosis, using crude BLV-preparation as antigen. This test is more sensitive than the immunodiffusion test and at least as sensitive as direct ELISA.
AuthorsC Platzer, H Siakkou, G Kraus, C Gröbel, S Rosenthal
JournalArchiv für experimentelle Veterinärmedizin (Arch Exp Veterinarmed) Vol. 44 Issue 6 Pg. 917-23 ( 1990) ISSN: 0003-9055 [Print] GERMANY
PMID1966364 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Viral Proteins
Topics
  • Animals
  • Antibodies, Monoclonal (immunology)
  • Cattle
  • Cattle Diseases (diagnosis)
  • Enzyme-Linked Immunosorbent Assay
  • Leukemia (diagnosis, veterinary)
  • Leukemia Virus, Bovine (immunology)
  • Predictive Value of Tests
  • Viral Proteins (immunology)

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