Abstract |
Using pentylenetetrazol (PTZ) in subconvulsant doses, it was found that the semisynthetic diterpene sclareol glycol (SG) from the labdane family of diterpenes at a dose well below the lethal dose, although not being convulsant, had a proconvulsant action in mice. This action is indicated by the number of mice undergoing convulsions and the reduction of latency. Specific binding of [3H]- diazepam in vitro to whole homogenate fractions prepared from hippocampus of rats was not inhibited by SG. At the same time comparatively investigated Ro 15-1788 inhibited [3H]- diazepam binding. It is suggested that the proconvulsant effect of SG is realized not through interactions with central benzodiazepine receptors as Ro 15-1788 does, but perhaps through interactions with adenylate cyclase (stimulating its catalytic subunit and thus increasing brain 3',5'- AMP availability). Other brain neurotransmitter systems (e.g., GABA) should also participate in realization of the proconvulsant effect of SG.
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Authors | J Georgieva, N Danchev |
Journal | Methods and findings in experimental and clinical pharmacology
(Methods Find Exp Clin Pharmacol)
Vol. 12
Issue 10
Pg. 679-83
(Dec 1990)
ISSN: 0379-0355 [Print] Spain |
PMID | 1966111
(Publication Type: Journal Article)
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Chemical References |
- Diterpenes
- Receptors, GABA-A
- Flumazenil
- Pentylenetetrazole
- sclareol glycol
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Topics |
- Animals
- Diterpenes
(metabolism, pharmacology)
- Flumazenil
(metabolism, pharmacology)
- Hippocampus
(metabolism)
- In Vitro Techniques
- Male
- Mice
- Pentylenetetrazole
- Radioligand Assay
- Rats
- Rats, Inbred Strains
- Receptors, GABA-A
(drug effects, metabolism)
- Seizures
(chemically induced, physiopathology)
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