Abstract |
Polyhydroxyalkanoates are energy reserve polymers produced by bacteria to survive periods of starvation in natural habitats. Little is known about the ecology of polyhydroxyalkanoate-producing bacteria. To analyse the occurrence of this specific group on/in seven different plant species, a combined strategy containing culture-dependent and -independent methods was applied. Using microbial fingerprint techniques (single-strand conformation polymorphism analysis with specific primers for phaC gene encoding the key enzyme of the polyhydroxyalkanoate synthesis), a high number of bands were especially found for the rhizosphere. Furthermore, cluster analysis revealed plant species-specific communities. Isolation of bacteria, recognition of brightly refractile cytoplasmatic inclusions, lipophilic stainings and a PCR strategy targeted on the phaC gene were used as a culture-dependent strategy for the detection of polyhydroxyalkanoate-producing bacteria. Results again represent a high degree of plant specificity: the rhizosphere of sugar beet contained the highest number of positive strains. This was confirmed by quantitative PCR: the relative copy number of phaC was statistically and significantly enhanced in all rhizospheres in comparison with bulk soil. New polyhydroxyalkanoate-producing bacterial species were detected: for example, Burkholderia terricola, Lysobacter gummosus, Pseudomonas extremaustralis, Pseudomonas brassicacearum and Pseudomonas orientalis. Our results confirm the hypothesis that the rhizosphere is an interesting hidden reservoir for polyhydroxyalkanoate producers.
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Authors | Ilona Gasser, Henry Müller, Gabriele Berg |
Journal | FEMS microbiology ecology
(FEMS Microbiol Ecol)
Vol. 70
Issue 1
Pg. 142-50
(Oct 2009)
ISSN: 1574-6941 [Electronic] England |
PMID | 19656194
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Bacterial
- Polyhydroxyalkanoates
- Soil
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Topics |
- Bacteria
(genetics, growth & development, isolation & purification, metabolism)
- Biodiversity
- Cluster Analysis
- DNA, Bacterial
(genetics)
- Plants
(microbiology)
- Polyhydroxyalkanoates
(biosynthesis)
- Polymorphism, Single-Stranded Conformational
- Sequence Analysis, DNA
- Soil
(analysis)
- Soil Microbiology
- Species Specificity
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