Inhibitory effects of 3-imino-5-phenyl-3H-1,2-dithiole on poliovirus type 1 replication in vitro.

Abstract

The effect of 3-imino-5-phenyl-3H-1,2-dithiole (PDTI) on different steps of the replicative cycle of poliovirus type 1 in HEp-2 cells was studied. This compound inhibited the replication of poliovirus type 1 as shown by cytopathic effect and virus yield reduction. This inhibitory action was not due to a virucidal effect, nor did the cells to have been pretreated. Under one-step growth conditions 3-imino-5-phenyl-3H-1,2-dithiole caused the greatest inhibition if added within 1 h after poliovirus adsorption. [5-3H]uridine incorporation into RNA showed that PDTI reduced poliovirus RNA synthesis. In fact, in the presence of PDTI viral RNA synthesis was shut off completely at 2 h post infection, and at 4 h post infection viral RNA synthesis was reduced by 50%. The compound may have an inhibitory effect on the early transcriptional and/or replicative functions of the poliovirus genome.

• Authors: A Garozzo, M R Pinizzotto, P M Furneri, D Baratta, F Guerrera, A Castro, B Tornetta, E Geremia
• Journal: Antiviral research (Antiviral Res) 1990 Oct-Nov Vol. 14 Issue 4-5 Pg. 267-77 ISSN: 0166-3542 [Print] Netherlands
• PMID: 1965112 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• DNA, Viral
• Imines
• RNA, Viral
• 3-imino-5-phenyl-3H-1,2-dithiole
• Thymidine
• Uridine

Topics

• Antiviral Agents (administration & dosage, pharmacology)
• Cells, Cultured
• Cytopathogenic Effect, Viral
• DNA, Viral (biosynthesis)
• Dose-Response Relationship, Drug
• Humans
• Imines (pharmacology)
• Poliomyelitis (drug therapy)
• Poliovirus (drug effects, genetics, growth & development)
• RNA, Viral (biosynthesis)
• Thymidine (genetics)
• Uridine (genetics)
• Viral Plaque Assay
• Virus Replication (drug effects)