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[B-type natriuretic peptide (BNP) levels and the effects of carvedilol on BNP levels in juvenile rats with right heart failure].

AbstractOBJECTIVE:
To examine serum B-type natriuretic peptide (BNP) levels and BNP expression of protein and mRNA in the right ventricular myocardium in juvenile rats with right heart failure (RHF) and the effects of beta-adrenergic receptor blocker carvedilol on serum and myocardial BNP levels in order to investigate the role of BNP in the diagnosis and treatment of RHF.
METHODS:
Fifty-one four-week-old Sprague-Dawley rats were randomly assigned to 5 groups: RHF 1, RHF 2, carvedilol-treated RHF, control 1 and control 2. RHF was developed 4 weeks after an intraperitoneal injection of monocrotaline in the RHF 1, RHF 2 and carvedilol-treated RHF groups. The rats in the RHF 1 and the control 1 groups were sacrificed after the RHF event for observing pathological changes in the myocardium. After the RHF event, the carvedilol-treated group was given intragastric administration of carvedilol (3.5 mg/kg/d) for 2 weeks. The RHF 2 and the control 2 groups were given distilled water of equal dose instead. The rats were sacrificed 2 weeks after carvedilol or distilled water administration. Serum BNP levels were measured using ELISA. BNP protein and mRNA expression in the right ventricular myocardium were measured by immunohistochemistry and RT-PCR, respectively. Haemodynamics and some physiological indexes were measured.
RESULTS:
Serum BNP levels and BNP protein and mRNA expression in the right ventricular myocardium were significantly higher in the RHF 1 group than those in the control 1 group (p<0.01). Serum BNP levels and BNP protein and mRNA expression in the right ventricular myocardium increased more significantly in the RHF 2 group. There was a positive correlation between serum BNP levels and myocardial BNP protein expression in the RHF group (r=0.698, p<0.01). Serum BNP levels and BNP protein and mRNA expression in the carvedilol-treated RHF group were significantly reduced when compared with the RHF 2 group (p<0.05). Carvedilol treatment also resulted in improved hemodynamics and relieved right ventricular hypertrophy.
CONCLUSIONS:
BNP may serve an index for the diagnosis of RHF and the evaluation of severity in children with RHF. Carvedilol shows protections against RHF caused by pressure load.
AuthorsJin-Dou An, Fang Liang, Song Feng
JournalZhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics (Zhongguo Dang Dai Er Ke Za Zhi) Vol. 11 Issue 7 Pg. 571-6 (Jul 2009) ISSN: 1008-8830 [Print] China
PMID19650993 (Publication Type: Journal Article)
Chemical References
  • Adrenergic beta-Antagonists
  • Carbazoles
  • Propanolamines
  • RNA, Messenger
  • Carvedilol
  • Natriuretic Peptide, Brain
Topics
  • Adrenergic beta-Antagonists (pharmacology)
  • Animals
  • Carbazoles (pharmacology, therapeutic use)
  • Carvedilol
  • Heart Failure (blood, drug therapy, pathology, physiopathology)
  • Natriuretic Peptide, Brain (blood, genetics)
  • Propanolamines (pharmacology, therapeutic use)
  • RNA, Messenger (analysis)
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley

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