Abstract | OBJECTIVE: METHODS: Fifty-one four-week-old Sprague-Dawley rats were randomly assigned to 5 groups: RHF 1, RHF 2, carvedilol-treated RHF, control 1 and control 2. RHF was developed 4 weeks after an intraperitoneal injection of monocrotaline in the RHF 1, RHF 2 and carvedilol-treated RHF groups. The rats in the RHF 1 and the control 1 groups were sacrificed after the RHF event for observing pathological changes in the myocardium. After the RHF event, the carvedilol-treated group was given intragastric administration of carvedilol (3.5 mg/kg/d) for 2 weeks. The RHF 2 and the control 2 groups were given distilled water of equal dose instead. The rats were sacrificed 2 weeks after carvedilol or distilled water administration. Serum BNP levels were measured using ELISA. BNP protein and mRNA expression in the right ventricular myocardium were measured by immunohistochemistry and RT-PCR, respectively. Haemodynamics and some physiological indexes were measured. RESULTS: Serum BNP levels and BNP protein and mRNA expression in the right ventricular myocardium were significantly higher in the RHF 1 group than those in the control 1 group (p<0.01). Serum BNP levels and BNP protein and mRNA expression in the right ventricular myocardium increased more significantly in the RHF 2 group. There was a positive correlation between serum BNP levels and myocardial BNP protein expression in the RHF group (r=0.698, p<0.01). Serum BNP levels and BNP protein and mRNA expression in the carvedilol-treated RHF group were significantly reduced when compared with the RHF 2 group (p<0.05). Carvedilol treatment also resulted in improved hemodynamics and relieved right ventricular hypertrophy. CONCLUSIONS: BNP may serve an index for the diagnosis of RHF and the evaluation of severity in children with RHF. Carvedilol shows protections against RHF caused by pressure load.
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Authors | Jin-Dou An, Fang Liang, Song Feng |
Journal | Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
(Zhongguo Dang Dai Er Ke Za Zhi)
Vol. 11
Issue 7
Pg. 571-6
(Jul 2009)
ISSN: 1008-8830 [Print] China |
PMID | 19650993
(Publication Type: Journal Article)
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Chemical References |
- Adrenergic beta-Antagonists
- Carbazoles
- Propanolamines
- RNA, Messenger
- Carvedilol
- Natriuretic Peptide, Brain
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Topics |
- Adrenergic beta-Antagonists
(pharmacology)
- Animals
- Carbazoles
(pharmacology, therapeutic use)
- Carvedilol
- Heart Failure
(blood, drug therapy, pathology, physiopathology)
- Natriuretic Peptide, Brain
(blood, genetics)
- Propanolamines
(pharmacology, therapeutic use)
- RNA, Messenger
(analysis)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
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